In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection
文献类型：期刊论文
| 作者 | Zhang, Fa-Li1; Yang, Liu3; He, Wei-Hao1; Xie, Ling-Jun1; Yang, Fei2; Wang, Ying-Hui3; Huang, Ai-Guo1 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2023-06-09 |
| 页码 | 10 |
| ISSN号 | 0140-7775 |
| 关键词 | antibacterial activity medicinal plants Sophora flavescens Streptococcus agalactiae tilapia |
| DOI | 10.1111/jfd.13818 |
| 通讯作者 | Wang, Ying-Hui(wyh@gxu.edu.cn) ; Huang, Ai-Guo(aiguo_200891@126.com) |
| 英文摘要 | Streptococcosis disease caused by Streptococcus agalactiae (Group B Streptococcus, GBS) results in a huge economic loss of tilapia culture. It is urgent to find new antimicrobial agents against streptococcosis. In this study, 20 medicinal plants were evaluated in vitro and in vivo to obtain medicinal plants and potential bioactive compounds against GBS infection. The results showed that the ethanol extracts of 20 medicinal plants had low or no antibacterial properties in vitro, with a minimal inhibitory concentration >= 256 mg/L. Interestingly, in vivo tests showed that 7 medicinal plants could significantly inhibit GBS infection in tilapia, and Sophora flavescens (SF) had the strongest anti-GBS activity in tilapia, reaching 92.68%. SF could significantly reduce the bacterial loads of GBS in different tissues (liver, spleen and brain) of tilapia after treated with different tested concentrations (12.5, 25.0, 50.0 and 100.0 mg/kg) for 24 h. Moreover, 50 mg/kg SF could significantly improve the survival rate of GBS-infected tilapia by inhibiting GBS replication. Furthermore, the expression of antioxidant gene cat, immune-related gene c-type lysozyme and anti-inflammatory cytokine il-10 in liver tissue of GBS-infected tilapia significantly increased after treated with SF for 24 h. Meanwhile, SF significantly reduced the expression of immune-related gene myd88 and pro-inflammatory cytokines il-8 and il-1 beta in liver tissue of GBS-infected tilapia. The negative and positive models of UPLC-QE-MS, respectively, identified 27 and 57 components of SF. The major components of SF extract in the negative model were alpha, alpha-trehalose, DL-malic acid, D-(-)-fructose and xanthohumol, while in the positive model were oxymatrine, formononetin, (-)-maackiain and xanthohumol. Interestingly, oxymatrine and xanthohumol could significantly inhibit GBS infection in tilapia. Taken together, these results suggest that SF can inhibit GBS infection in tilapia, and it has potential for the development of anti-GBS agents. |
| WOS关键词 | DISEASE RESISTANCE ; TILAPIA |
| 资助项目 | National Natural Science Foundation of China[42106133] ; Science and Technology Project of Guangxi[AD22080029] ; Natural Science Foundation of Guangxi[2021GXNSFBA075008] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:001005183400001 |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Project of Guangxi ; Natural Science Foundation of Guangxi |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/194900]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Wang, Ying-Hui; Huang, Ai-Guo |
| 作者单位 | 1.Guangxi Univ, Sch Marine Sci, Guangxi Lab Study Coral Reefs South China Sea, Nanning 530004, Peoples R China 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, State Key Lab Resources & Environm Informat Syst, Beijing, Peoples R China 3.Guangxi lnst lndustrial Technol, Inst Green & Low Carbon Technol, Nanning 530004, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Fa-Li,Yang, Liu,He, Wei-Hao,et al. In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection[J]. JOURNAL OF FISH DISEASES,2023:10. |
| APA | Zhang, Fa-Li.,Yang, Liu.,He, Wei-Hao.,Xie, Ling-Jun.,Yang, Fei.,...&Huang, Ai-Guo.(2023).In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection.JOURNAL OF FISH DISEASES,10. |
| MLA | Zhang, Fa-Li,et al."In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection".JOURNAL OF FISH DISEASES (2023):10. |
入库方式： OAI收割
来源：地理科学与资源研究所
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