Exopolysaccharides of Serratia fonticola CPSE11 can alleviate the toxic effect of Cd(2+)on Codonopsis pilosula
文献类型：期刊论文
| 作者 | Leng, Feifan1; Zhang, Kexin1; Hu, Shu1; Li, Shaowei2; Yu, Chengqun2; Wang, Yonggang1 |
| 刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
 |
| 出版日期 | 2023-06-10 |
| 页码 | 15 |
| ISSN号 | 0944-1344 |
| 关键词 | Serratia fonticola CPSE11 Exopolysaccharides Codonopsis pilosula Cd2+ Hydroponic experiment |
| DOI | 10.1007/s11356-023-28145-0 |
| 通讯作者 | Wang, Yonggang(412316788@163.com) |
| 英文摘要 | In order to study the detoxification effect of microbial exopolysaccharides (EPS) on the heavy metal cadmium (Cd2+), this study took an EPS-producing Serratia fonticola CPSE11 (NZ_CP050171.1) isolated from Codonopsis pilosula root as the research object. The whole genome and EPS synthesis gene clusters of this strain were predicted and analyzed, the adsorption kinetics of EPS on Cd2+ were studied by using pseudo-first-order and second-order kinetic equations, the isothermal adsorption curves were simulated and analyzed by using the Langmuir isothermal adsorption equation, and the effects of Cd2+ and EPS on the growth of C. pilosula were explored by seed germination experiment and hydroponic experiment. The analysis revealed that this strain contained three gene clusters related to EPS synthesis, and the metabolic pathway for EPS synthesis was obtained on the basis of the whole genome analysis and microbial physiological metabolism. The molecular weight and monosaccharide composition of EPS were determined by HPLC analysis, which showed that EPS consisted of mannose, glucosamine, rhamnose, galactosamine, glucose, and galactose with a molar ratio of 1:1.74:4.57:3.96:14.04:10.28, with the molecular weight of 366,316.09 kDa. The adsorption process of EPS on Cd2+ was in accordance with the second-order kinetic model, and the results of seed germination experiments showed that EPS could promote seed germination and improve seed activity. In the hydroponic experiment, high concentration of Cd2+ (15 mg/L) caused toxic symptoms in C. pilosula, while the addition of EPS reduced the toxic effect of Cd2+ on C. pilosula, and the plant growth was significantly improved. |
| WOS关键词 | EXTRACELLULAR POLYMERIC SUBSTANCES ; PLANT-GROWTH ; MONOSACCHARIDE COMPOSITION ; BIOFILM FORMATION ; HEAVY-METAL ; PSEUDOMONAS ; BACTERIAL ; POLYSACCHARIDES ; BIOSYNTHESIS ; ANTIOXIDANT |
| 资助项目 | National Natural Science Foundation of China[31760028] ; Major Science and Technology Project of Tibet[XZ201801NA02] ; Youth Talent Support Program of Lanzhou University of Technology[2018] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:001005505100002 |
| 资助机构 | National Natural Science Foundation of China ; Major Science and Technology Project of Tibet ; Youth Talent Support Program of Lanzhou University of Technology |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/194914]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Wang, Yonggang |
| 作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modelling, Natl Ecol Res Stn, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Leng, Feifan,Zhang, Kexin,Hu, Shu,et al. Exopolysaccharides of Serratia fonticola CPSE11 can alleviate the toxic effect of Cd(2+)on Codonopsis pilosula[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2023:15. |
| APA | Leng, Feifan,Zhang, Kexin,Hu, Shu,Li, Shaowei,Yu, Chengqun,&Wang, Yonggang.(2023).Exopolysaccharides of Serratia fonticola CPSE11 can alleviate the toxic effect of Cd(2+)on Codonopsis pilosula.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,15. |
| MLA | Leng, Feifan,et al."Exopolysaccharides of Serratia fonticola CPSE11 can alleviate the toxic effect of Cd(2+)on Codonopsis pilosula".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023):15. |
入库方式： OAI收割
来源：地理科学与资源研究所
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