Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir
文献类型：期刊论文
| 作者 | Guo, Bin2; Stiles, Amanda R.2,3; Liu, Chun-Zhao1,2 |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 48期号:6页码:609-612 |
| 关键词 | Saussurea involucrata Shoot organogenesis Thidiazuron Germplasm conservation |
| ISSN号 | 1054-5476 |
| 通讯作者 | Liu, CZ |
| 英文摘要 | An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of culture, 15.6 +/- 1.4 shoots were regenerated per leaf explant on MS medium containing 0.5 mu M TDZ. After transfer of shoots to a medium containing 5.0 mu M indole-3-acetic acid, approximately 80% of the regenerated shoots formed roots and whole plantlets. After transfer of rooted shoots to the greenhouse, 83% of the regenerated plantlets survived and grew vigorously. The regeneration protocol developed in this study provides a basis for germplasm conservation and for the production of plant material necessary to study the medicinally active components of S. involucrata. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 关键词[WOS] | ARTEMISIA-JUDAICA L ; PLANT-REGENERATION ; TISSUE-CULTURE ; MICROPROPAGATION ; CONSERVATION ; SEEDLINGS ; SEGMENTS ; GROWTH ; L. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312560000004 |
| 公开日期 | 2013-10-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2947]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA |
| 推荐引用方式 GB/T 7714 | Guo, Bin,Stiles, Amanda R.,Liu, Chun-Zhao. Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2012,48(6):609-612. |
| APA | Guo, Bin,Stiles, Amanda R.,&Liu, Chun-Zhao.(2012).Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,48(6),609-612. |
| MLA | Guo, Bin,et al."Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 48.6(2012):609-612. |
入库方式： OAI收割
来源：过程工程研究所
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