Oriented immobilization of glucose oxidase on graphene oxide
文献类型：期刊论文
| 作者 | Zhou, Liya1,2; Jiang, Yanjun1,2; Gao, Jing1; Zhao, Xinqiang1; Ma, Li1; Zhou, Qianlin1 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2012-12-15 |
| 卷号 | 69期号:0页码:28-31 |
| 关键词 | Immobilized enzymes Affinity Glucose Graphene oxide Glucose oxidase Kinetic parameters |
| ISSN号 | 1369-703X |
| 通讯作者 | Gao, J |
| 英文摘要 | The oriented immobilization of glucose oxidase (GOD) on a selective support composed of graphene oxide (GO) and concanavalin A (Con A) was prepared in this study. Specially, the amino groups of Con A were covalently attached to the carboxyl groups of pre-activated GO, and then GOD was oriented immobilized on the GO-Con A conjugate via strong biospecific affinity between the sugar residues of GOD and Con A. Compared with free GOD and randomly immobilized GOD (GO-GOD), the high-affinity of Con A and GOD endowed the oriented immobilized GOD (GO-Con A-GOD) with a wider pH stability range, a better thermal stability, a longer-term storage stability and a higher resistance ability toward the denaturing agents. The Michaelis constant (K-m) of GO-Con A-GOD was very close to that of free GOD. Hopefully, the GO-Con A-GOD may be further integrated into functional bioelectrodes for biosensor applications. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | CONCANAVALIN-A ; NANOPARTICLES ; INACTIVATION ; MECHANISM ; POLYMER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312178500004 |
| 公开日期 | 2013-10-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2952]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Liya,Jiang, Yanjun,Gao, Jing,et al. Oriented immobilization of glucose oxidase on graphene oxide[J]. BIOCHEMICAL ENGINEERING JOURNAL,2012,69(0):28-31. |
| APA | Zhou, Liya,Jiang, Yanjun,Gao, Jing,Zhao, Xinqiang,Ma, Li,&Zhou, Qianlin.(2012).Oriented immobilization of glucose oxidase on graphene oxide.BIOCHEMICAL ENGINEERING JOURNAL,69(0),28-31. |
| MLA | Zhou, Liya,et al."Oriented immobilization of glucose oxidase on graphene oxide".BIOCHEMICAL ENGINEERING JOURNAL 69.0(2012):28-31. |
入库方式： OAI收割
来源：过程工程研究所
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