Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots
文献类型：期刊论文
| 作者 | Abbasi, Bilal H.2; Stiles, Amanda R.2; Saxena, Praveen K.3,4; Liu, Chun-Zhao1,2 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 168期号:7页码:2057-2066 |
| 关键词 | Echinacea purpurea Hairy roots Cichoric acid Anthocyanins GA(3) Light |
| ISSN号 | 0273-2289 |
| 通讯作者 | Liu, CZ |
| 英文摘要 | Gibberellic acid (GA(3)) is reported to have diverse effects on hairy root cultures of many plant species; therefore, the effects of GA(3) on the growth, secondary metabolite production (caffeic acid derivatives and lignin), phenylalanine ammonia lyase (PAL) activity, and free radical scavenging activity of light-grown Echinacea purpurea L. hairy roots were investigated. Eight concentrations of GA(3), ranging from 0.005 to 1.0 mu M, were added to shake flask cultures. The moderate GA(3) concentration, 0.025 mu M, resulted in the highest concentrations of cichoric acid, caftaric acid, and chlorogenic acid, as well as increased PAL activity, cell viability, and free radical scavenging activity, while higher and lower GA(3) concentrations resulted in reduced levels compared to the control (lacking GA(3)). The moderate GA(3) concentration also affected root morphogenesis; supplementation with 0.025 mu M GA(3) resulted in the development of thick, dense, purple-colored roots, while roots exposed to the higher and lower concentrations of GA(3) were thin and off-white. This study demonstrates that supplementation with GA(3) may be an excellent strategy to optimize the production of secondary metabolites from E. purpurea hairy root cultures; however, the GA(3) concentration is a critical factor. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CICHORIUM-INTYBUS L. ; PHENOLIC-COMPOUNDS ; CULTURES ; GROWTH ; ANTIOXIDANT ; DERIVATIVES ; TISSUE ; GA(3) ; BIOSYNTHESIS ; INHIBITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312205000025 |
| 公开日期 | 2013-10-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2954]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Univ Guelph, Gosling Res Inst Plant Preservat, Guelph, ON N1G 2W1, Canada 4.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada |
| 推荐引用方式 GB/T 7714 | Abbasi, Bilal H.,Stiles, Amanda R.,Saxena, Praveen K.,et al. Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,168(7):2057-2066. |
| APA | Abbasi, Bilal H.,Stiles, Amanda R.,Saxena, Praveen K.,&Liu, Chun-Zhao.(2012).Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,168(7),2057-2066. |
| MLA | Abbasi, Bilal H.,et al."Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 168.7(2012):2057-2066. |
入库方式： OAI收割
来源：过程工程研究所
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