Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation
文献类型：期刊论文
| 作者 | Xu, Xia1; Liu, Yang2; Cui, Zhanfeng3; Wei, Yuping1; Zhang, Liang1 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2012-12-31 |
| 卷号 | 162期号:2-3页码:224-231 |
| 关键词 | Human mesenchymal stem cells Cryopreservation Osmotic shock Cold shock Adherent cells |
| ISSN号 | 0168-1656 |
| 通讯作者 | Xu, X |
| 英文摘要 | Cryopreservation is one of the most practical methods for the long-term storage of cell-matrix systems to ensure off-shelf availability in tissue engineering, stem cell therapy and drug testing. The aim of this study is to investigate the effects of osmotic and cold shock caused by the procedures of cryoprotectant agent addition/removal and freezing during cryopreservation on cell viability, intracellular properties, such as filamentous actin distribution, mitochondria localization and intracellular pH, and further recovery of adherent human mesenchymal stem cells. Our results shows a significant decrease in cell viability around 30% after cryopreservation at the cooling rates of 1, 5 and 10 degrees C/min in comparison to the adherent cells and the cells in suspension, implicating that the adherent cells are more vulnerable than the suspension cells. The osmotic shock and cold shock induced by freezing lead to dramatic changes in the intracellular properties. The cooling rate of 10 degrees C/min results in acidification of intracellular pH, distortion and accumulation of filamentous actin, and aggregation of mitochondria. Our findings also suggest that the cooling rate of 1 degrees C/min helps to maintain cell morphology and attachment, integrity and uniformity of filamentous actin, and leads to better cell recovery after cryopreservation. (C) 2012 Elsevier B. V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BONE-MARROW ; IN-VITRO ; DIFFERENTIATION ; CULTURES ; TRANSPLANTATION ; PROLIFERATION ; CYTOSKELETON ; PROGENITORS ; VIABILITY ; RECOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311642000007 |
| 公开日期 | 2013-10-10 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3138]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Regenerat Med Ctr, Dalian, Peoples R China 3.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England |
| 推荐引用方式 GB/T 7714 | Xu, Xia,Liu, Yang,Cui, Zhanfeng,et al. Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation[J]. JOURNAL OF BIOTECHNOLOGY,2012,162(2-3):224-231. |
| APA | Xu, Xia,Liu, Yang,Cui, Zhanfeng,Wei, Yuping,&Zhang, Liang.(2012).Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation.JOURNAL OF BIOTECHNOLOGY,162(2-3),224-231. |
| MLA | Xu, Xia,et al."Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation".JOURNAL OF BIOTECHNOLOGY 162.2-3(2012):224-231. |
入库方式： OAI收割
来源：过程工程研究所
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