Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter
文献类型：期刊论文
| 作者 | Wang, Ziqiang1,2; Wang, Yunshan1; Shi, Hong1; Su, Zhiguo1 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2012-12-31 |
| 卷号 | 162期号:2-3页码:232-236 |
| 关键词 | cis-Epoxysuccinate hydrolase PLPR promoter Prokaryotic expression Scale-up |
| ISSN号 | 0168-1656 |
| 通讯作者 | Wang, YS |
| 英文摘要 | cis-Epoxysuccinate hydrolase (ESH) from Nocardia tartaricans CAS-52 could catalyze the stereospecific hydrolysis of cis-epoxysuccinate to L-(+)-tartrate. The ESH gene of 762 bp was cloned and its open reading frame (ORF) sequence predicted a protein of 253 amino acids. An expression plasmid carrying the ESH gene under the control of the PLPR promoter was introduced into Escherichia coli, and the ESH gene was successfully expressed in the recombinant strain. The expression conditions and scale-up production were also studied. Fed-batch fermentation of E. coli Trans 1-T1 transformant was carried out in a 2000 L fermentor to product recombinant ESH. The results showed that wet cell concentration reached to 62.45 g L-1, and the specific activity of ESH was 380.17 U mg(-1), which could meet the requirements of industrial production of L-(+)-tartaric acid. (C) 2012 Elsevier B. V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | EPOXIDE HYDROLASE ; TARTARIC ACID ; NOCARDIA-TARTARICANS ; CELLS ; MECHANISM ; PROTEINS ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311642000008 |
| 公开日期 | 2013-10-10 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3139]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ziqiang,Wang, Yunshan,Shi, Hong,et al. Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter[J]. JOURNAL OF BIOTECHNOLOGY,2012,162(2-3):232-236. |
| APA | Wang, Ziqiang,Wang, Yunshan,Shi, Hong,&Su, Zhiguo.(2012).Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter.JOURNAL OF BIOTECHNOLOGY,162(2-3),232-236. |
| MLA | Wang, Ziqiang,et al."Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter".JOURNAL OF BIOTECHNOLOGY 162.2-3(2012):232-236. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。