Immobilizing Penicillin G Acylase Using Silica-Supported Ionic Liquids: The Effects of Ionic Liquid Loadings
文献类型：期刊论文
| 作者 | Zhou, Huacong1,2; Yang, Liangrong1; Li, Wei3; Wang, Fuchun1,2; Li, Wensong1,2 ; Zhao, Junmei1; Liang, Xiangfeng1; Liu, Huizhou1
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| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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| 出版日期 | 2012-10-10 |
| 卷号 | 51期号:40页码:13173-13181 |
| 关键词 | CARBON-DIOXIDE RAPID SYNTHESIS NANOPARTICLES STABILITY CATALYSIS LIPASE PHASE |
| ISSN号 | 0888-5885 |
| 通讯作者 | Yang, LR |
| 英文摘要 | The supported ionic liquids (SILs) were prepared via immobilizing silane-functionalized ionic liquids (IL) onto the monodispersive silica nanoparticles, and the composite material was then applied in the immobilization of penicillin G acylase (PGA, EC 3.5.1.11), an important industrial enzyme for the production of semisynthetic antibiotics. This was a novel approach of immobilizing PGA proposed in our previous report, in which we had studied the effects of IL types on the performance of this novel support. Here, we focused on the effects of IL loading on the properties of the SILs and their performance in PGA immobilization. The effects of silica sizes and routes of preparing SILs (the solvent route and the sol-gel route) on IL loading were investigated. The results showed that (1) the IL loading could be tuned efficiently by changing the silica sizes. (2) As compared with the solvent route, the sol-gel route is more simple, time-saving, and ecofriendly in operation. What's more, it has a higher IL loading. Among the factors affecting the IL loading, the reaction time was the most feasible factor to control to tune the IL loading, as compared with the temperature and the dosage of ILs. (3) With the increase of IL loading, the surface zeta-potential of silicas changed from negative to positive, and the isoelectronic point (IEP) increased accordingly, which caused the decrease of the protein loading but the increase of apparent and specific activity. A reusability experiment showed that the sol-gel 1 sample maintained approximately 66% of the initial activity even after 10 consecutive operation cycles under the experimental conditions. This activity was 3.7 times higher than that of the sol-gel 2 sample. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | CARBON-DIOXIDE ; RAPID SYNTHESIS ; NANOPARTICLES ; STABILITY ; CATALYSIS ; LIPASE ; PHASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309624500021 |
| 公开日期 | 2013-10-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3186]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Huacong,Yang, Liangrong,Li, Wei,et al. Immobilizing Penicillin G Acylase Using Silica-Supported Ionic Liquids: The Effects of Ionic Liquid Loadings[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2012,51(40):13173-13181. |
| APA | Zhou, Huacong.,Yang, Liangrong.,Li, Wei.,Wang, Fuchun.,Li, Wensong.,...&Liu, Huizhou.(2012).Immobilizing Penicillin G Acylase Using Silica-Supported Ionic Liquids: The Effects of Ionic Liquid Loadings.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,51(40),13173-13181. |
| MLA | Zhou, Huacong,et al."Immobilizing Penicillin G Acylase Using Silica-Supported Ionic Liquids: The Effects of Ionic Liquid Loadings".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 51.40(2012):13173-13181. |
入库方式： OAI收割
来源：过程工程研究所
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