Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support
文献类型：期刊论文
| 作者 | Qu, Jian-Bo1; Shao, Hui-Hui1; Li, Juan2; Jing, Guang-Lun1; Liu, Jian-Guo1; Zhou, Wei-Qing2 |
| 刊名 | REACTIVE & FUNCTIONAL POLYMERS
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 72期号:9页码:606-612 |
| 关键词 | Gigaporous Hydrophilization Polystyrene microspheres Flow hydrodynamics Protein adsorption |
| ISSN号 | 1381-5148 |
| 通讯作者 | Qu, JB |
| 英文摘要 | Gigaporous poly(styrene-divinylbenzene) (PS) microspheres were hydrophilically modified with natural saccharide to minimize their nonspecific adsorption to proteins. The microspheres were chloroacetylated through Friedel-Crafts acetylation with chloroacetyl chloride, and then coupled with diacetone-D-glucose (DAGlu) through the Williamson reaction, and the protecting groups were removed on DAGlu. Results showed that the PS microspheres were successfully coupled with DAGlu and that the gigaporous structure was well maintained. After hydrophilization (Glu-PS), nonspecific adsorption of proteins on PS microspheres was greatly reduced. The high surface density of hydroxyl groups on Glu-PS microspheres surface make it easy to derivatize the spheres by classical methods. Flow experiments showed that the Glu-PS column had low backpressure, good permeability, and mechanical stability. All results indicate that the Glu-PS microspheres have great potential applications in high-speed protein chromatography. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Applied ; Engineering, Chemical ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Engineering ; Polymer Science |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; EXCHANGE PACKING MATERIAL ; PERFUSION CHROMATOGRAPHY ; STATIONARY-PHASE ; ADSORPTION ; SEPARATION ; PARTICLES ; DEXTRAN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307695200008 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3227]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Jian-Bo,Shao, Hui-Hui,Li, Juan,et al. Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support[J]. REACTIVE & FUNCTIONAL POLYMERS,2012,72(9):606-612. |
| APA | Qu, Jian-Bo,Shao, Hui-Hui,Li, Juan,Jing, Guang-Lun,Liu, Jian-Guo,&Zhou, Wei-Qing.(2012).Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support.REACTIVE & FUNCTIONAL POLYMERS,72(9),606-612. |
| MLA | Qu, Jian-Bo,et al."Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support".REACTIVE & FUNCTIONAL POLYMERS 72.9(2012):606-612. |
入库方式： OAI收割
来源：过程工程研究所
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