Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation
文献类型：期刊论文
| 作者 | Han, Yejun; Chen, Hongzhang |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 99期号:14页码:6081-6087 |
| 关键词 | beta-glucosidase characterization optimum temperatures corn stover simultaneous saccharification and fermentation (SSF) |
| ISSN号 | 0960-8524 |
| 其他题名 | Bioresour. Technol. |
| 中文摘要 | Purification and characterization of beta-glucosidase from corn stover was performed and the enzyme was tried in SSF to evaluate the suitability of plant glycosyl hydrolases in lignocellulose conversion. A beta-glucosidase with M(w) of 62.4 kDa was purified to homogeneity from post-harvest corn stover. The following physicochemical and kinetic parameters of the beta-glucosidase were studied respectively: optimum temperature, thermal stability, optimum pH, pH stability, K(m), V(max), V(i), cellobiose inhibition, tryptic peptide mass spectrometry and effect of metal ions and other reagents on the activity. The beta-glucosidase activity on salicin was optimal at pH 4.8 and 37 degrees C. The unique property of optimum temperature makes the beta-glucosidase potentially useful in SSF. In SSF of steam explosion pretreated corn stover, the supplementation of the purified beta-glucosidase was more effective than Aspergillus niger beta-glucosidase. (C) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Purification and characterization of beta-glucosidase from corn stover was performed and the enzyme was tried in SSF to evaluate the suitability of plant glycosyl hydrolases in lignocellulose conversion. A beta-glucosidase with M(w) of 62.4 kDa was purified to homogeneity from post-harvest corn stover. The following physicochemical and kinetic parameters of the beta-glucosidase were studied respectively: optimum temperature, thermal stability, optimum pH, pH stability, K(m), V(max), V(i), cellobiose inhibition, tryptic peptide mass spectrometry and effect of metal ions and other reagents on the activity. The beta-glucosidase activity on salicin was optimal at pH 4.8 and 37 degrees C. The unique property of optimum temperature makes the beta-glucosidase potentially useful in SSF. In SSF of steam explosion pretreated corn stover, the supplementation of the purified beta-glucosidase was more effective than Aspergillus niger beta-glucosidase. (C) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000257150800011 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2759]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yejun,Chen, Hongzhang. Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation[J]. BIORESOURCE TECHNOLOGY,2008,99(14):6081-6087. |
| APA | Han, Yejun,&Chen, Hongzhang.(2008).Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation.BIORESOURCE TECHNOLOGY,99(14),6081-6087. |
| MLA | Han, Yejun,et al."Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation".BIORESOURCE TECHNOLOGY 99.14(2008):6081-6087. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。