Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system
文献类型：期刊论文
| 作者 | Jiang, Yangyang1,2; Xia, Hansong1; Guo, Chen1; Mahmood, Iram1,2; Liu, Huizhou1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 144期号:2页码:145-159 |
| 关键词 | three-liquid-phase system covalent attachment PEO-PPO-PEO polymer (L63) ionic liquids (Cyphos IL-104) penicillin G acylase 6-aminopenicillanic acid (6-APA) |
| ISSN号 | 0273-2289 |
| 其他题名 | Appl. Biochem. Biotechnol. |
| 中文摘要 | A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its "cloud point". 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40 degrees C) and stability (enzymatic half-time up to 55 h at 60 degrees C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product's side with 6-APA productivity of 80% at 42 degrees C, pH 5.5. |
| 英文摘要 | A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its "cloud point". 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40 degrees C) and stability (enzymatic half-time up to 55 h at 60 degrees C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product's side with 6-APA productivity of 80% at 42 degrees C, pH 5.5. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | AQUEOUS 2-PHASE SYSTEMS ; 6-AMINOPENICILLANIC ACID ; REACTIVE EXTRACTION ; G ACYLASE ; EQUILIBRIUM ; ANTIBIOTICS ; STABILITY ; KINETICS ; IMMOBILIZATION ; BIOCATALYST |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000252562900005 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2777]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Lab Seperat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yangyang,Xia, Hansong,Guo, Chen,et al. Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2008,144(2):145-159. |
| APA | Jiang, Yangyang,Xia, Hansong,Guo, Chen,Mahmood, Iram,&Liu, Huizhou.(2008).Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,144(2),145-159. |
| MLA | Jiang, Yangyang,et al."Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 144.2(2008):145-159. |
入库方式： OAI收割
来源：过程工程研究所
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