Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface
文献类型：期刊论文
| 作者 | Mahmood, Iram1,2; Guo, Chen1; Xia, Hansong1,2; Ma, Junhe1; Jiang, Yangyang1,2; Liu, Huizhou1 |
| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
 |
| 出版日期 | 2008-09-03 |
| 卷号 | 47期号:17页码:6379-6385 |
| 关键词 | aqueous-solutions enzymes oil aggregation surfactants crystals |
| ISSN号 | 0888-5885 |
| 其他题名 | Ind. Eng. Chem. Res. |
| 中文摘要 | Here, we have reported a new approach for utilizing oleic acid-Pluronic L-64 block copolymer coated iron oxide nanoparticles as supports for enzyme immobilization. Iron oxide nanoparticles were prepared by a coprecipitation method and were coated with oleic acid and Pluronic to achieve higher stability and dispersibility. The surface morphology and size of the particle, as determined by transmission electron microscopy (TEM), was +/- 10 nm. X-ray diffraction (XRD) patterns were taken over a range from 10 degrees to 90 degrees 20, using Cu K alpha radiation. Saturation magnetization values, measured at 300 K, varied from 34.6 emu/g to 60.8 emu/g. The possible interaction behavior of oleic acid and Pluronic was observed by Fourier transform infrared (FTIR) analysis and nuclear magnetic resonance (NMR) studies. Further potential of this material as a support for lipase immobilization and enzymatic hydrolysis at the oil/water interface was also investigated. The features of the surface-coated magnetic particles enable the adsorption of lipase from Candida cylindraces via strong hydrophobic interactions, which enhances the stability of the adsorbed enzyme molecules. The stability of the catalyst and, hence, its industrial applicability was tested by performing subsequent reaction cycles for the hydrolysis of olive oil. The activity of the immobilized lipase, pretreated with its substrate, was 510 U/g-matrix and was observed to be maintained at levels as high as 90% of its original activity for up to at least seven reuses. |
| 英文摘要 | Here, we have reported a new approach for utilizing oleic acid-Pluronic L-64 block copolymer coated iron oxide nanoparticles as supports for enzyme immobilization. Iron oxide nanoparticles were prepared by a coprecipitation method and were coated with oleic acid and Pluronic to achieve higher stability and dispersibility. The surface morphology and size of the particle, as determined by transmission electron microscopy (TEM), was +/- 10 nm. X-ray diffraction (XRD) patterns were taken over a range from 10 degrees to 90 degrees 20, using Cu K alpha radiation. Saturation magnetization values, measured at 300 K, varied from 34.6 emu/g to 60.8 emu/g. The possible interaction behavior of oleic acid and Pluronic was observed by Fourier transform infrared (FTIR) analysis and nuclear magnetic resonance (NMR) studies. Further potential of this material as a support for lipase immobilization and enzymatic hydrolysis at the oil/water interface was also investigated. The features of the surface-coated magnetic particles enable the adsorption of lipase from Candida cylindraces via strong hydrophobic interactions, which enhances the stability of the adsorbed enzyme molecules. The stability of the catalyst and, hence, its industrial applicability was tested by performing subsequent reaction cycles for the hydrolysis of olive oil. The activity of the immobilized lipase, pretreated with its substrate, was 510 U/g-matrix and was observed to be maintained at levels as high as 90% of its original activity for up to at least seven reuses. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | AQUEOUS-SOLUTIONS ; ENZYMES ; OIL ; AGGREGATION ; SURFACTANTS ; CRYSTALS |
| 收录类别 | ISTP ; SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000258777700008 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2821]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Lab Separat Sci & Engn, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mahmood, Iram,Guo, Chen,Xia, Hansong,et al. Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2008,47(17):6379-6385. |
| APA | Mahmood, Iram,Guo, Chen,Xia, Hansong,Ma, Junhe,Jiang, Yangyang,&Liu, Huizhou.(2008).Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,47(17),6379-6385. |
| MLA | Mahmood, Iram,et al."Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 47.17(2008):6379-6385. |
入库方式： OAI收割
来源：过程工程研究所
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