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Mannosylated engineered trichosanthin-legumain protein vaccine hydrogel for breast cancer immunotherapy

文献类型：期刊论文

作者Chen, Guihua1,2,3; Xiong, Wei1,2,3; Gu, Zeyun1,4; Gao, Yanrong1; Hou, Jiazhen5; Long, Li1,2,3; Wang, Huiyuan1; Asrorov, Akmal M.1,6; Muhitdinov, Bahtiyor1,6; Xu, Qin3
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2022-12-31
卷号223页码:1485-1494
关键词Engineered protein Hydrogel Cancer vaccine Trichosanthin Legumain Post-surgery
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2022.11.045
通讯作者Xu, Qin(xuqin@gzucm.edu.cn) ; Huang, Yongzhuo(yzhuang@simm.ac.cn)
英文摘要The development of cancer vaccines based on tumor-associated antigens is hurdled by lack of an efficient adjuvant and insufficient efficacy. To improve the efficacy of vaccines, a genetically-engineered method was employed in this work to achieve the codelivery of antigen and adjuvant to enhance immune responses. Trichosanthin is a plant-derived protein that possesses cancer immune stimulation function. A genetically engineered protein vaccine composed of trichosanthin (adjuvant) and legumain domain (a peptidic antigen) was constructed, which was further chemically modified with mannose for targeting dendritic cells (DCs). The method is facile and ready for scaling up for massive production. Such a "two-in-one" vaccine is advantageous for codelivery for augmenting the immune responses. The vaccine inhibited the tumors by triggering a robust cytotoxic T lymphocyte response in the orthotopic-breast-tumor mice. Furthermore, the vaccine was loaded into the temperature-sensitive hydrogel based on Pluronic F127 for implanting use in the post-surgical site. The sustained-released vaccine from the hydrogel inhibited not only the tumor recurrence but also the lung metastases of breast cancer. These findings demonstrated that it was a safe and effective vaccination for breast cancer immunotherapy in a prophylactical and therapeutical manner for remodeling the tumor immune microenvironment and arresting tumor growth.
WOS关键词MACROPHAGES
资助项目National Key Research and Development Program of China[2021YFE0103100] ; National Key Research and Development Program of China[2021YFC2400600] ; Chinese Pharmaceutical Association-Yiling Joint Project[CPAYLJ201901] ; NFSC[81925035] ; Shanghai SciTech Innovation Initiative[19431903100] ; Shanghai SciTech Innovation Initiative[18430740800]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001065363500001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/306125]  
专题新药研究国家重点实验室
通讯作者Xu, Qin; Huang, Yongzhuo
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Medica, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Inst Drug Discovery & Dev, Zhongshan 528437, Peoples R China
3.Guangzhou Univ Chinese Med, Artemisinin Res Ctr, 12 Jichang Rd, Guangzhou 510450, Peoples R China
4.Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macau, Peoples R China
5.Nanjing Univ Chinese Med, Sch Chinese Mat Medica, 138 Xianlin Ave,10, Nanjing 210023, Peoples R China
6.Acad Sci Uzbek, Inst Bioorgan Chem, 83 M Ulughbek St, Tashkent 100125, Uzbekistan
推荐引用方式
GB/T 7714		 Chen, Guihua,Xiong, Wei,Gu, Zeyun,et al. Mannosylated engineered trichosanthin-legumain protein vaccine hydrogel for breast cancer immunotherapy[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,223:1485-1494.
APA Chen, Guihua.,Xiong, Wei.,Gu, Zeyun.,Gao, Yanrong.,Hou, Jiazhen.,...&Huang, Yongzhuo.(2022).Mannosylated engineered trichosanthin-legumain protein vaccine hydrogel for breast cancer immunotherapy.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,223,1485-1494.
MLA Chen, Guihua,et al."Mannosylated engineered trichosanthin-legumain protein vaccine hydrogel for breast cancer immunotherapy".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 223(2022):1485-1494.

入库方式： OAI收割

来源：上海药物研究所

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