The claim of primacy of human gut Bacteroides ovatus in dietary cellobiose degradation
文献类型：期刊论文
| 作者 | Li, Meixia3; Wang, Yeqing3; Guo, Ciliang3,4; Wang, Sheng1; Zheng, Liangzhen1; Bu, Yifan1; Ding, Kan2,3,4,5
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| 刊名 | GUT MICROBES
 |
| 出版日期 | 2023-12-31 |
| 卷号 | 15期号:1页码:18 |
| ISSN号 | 1949-0976 |
| 关键词 | Human gut microbiota cellobiose bacteroide ovatus cellulase structure |
| DOI | 10.1080/19490976.2023.2227434 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | A demonstration of cellulose degrading bacterium from human gut changed our view that human cannot degrade the cellulose. However, investigation of cellulose degradation by human gut microbiota on molecular level has not been completed so far. We showed here, using cellobiose as a model that promoted the growth of human gut key members, such as Bacteroides ovatus (BO), to clarify the molecular mechanism. Our results showed that a new polysaccharide utilization locus (PUL) from BO was involved in the cellobiose capturing and degradation. Further, two new cellulases BACOVA_02626(GH5) and BACOVA_02630(GH5) on the cell surface performed the degradation of cellobiose into glucose were determined. The predicted structures of BACOVA_02626(GH5) and BACOVA_02630(GH5) were highly homologous with the cellulase from soil bacteria, and the catalytic residues were highly conservative with two glutamate residues. In murine experiment, we observed cellobiose reshaped the composition of gut microbiota and probably modified the metabolic function of bacteria. Taken together, our findings further highlight the evidence of cellulose can be degraded by human gut microbes and provide new insight in the field of investigation on cellulose. |
| WOS关键词 | CELLULOLYTIC BACTERIA ; MECHANISM ; CELLULASE ; REVEALS ; ENZYMES |
| WOS研究方向 | Gastroenterology & Hepatology ; Microbiology |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS INC |
| WOS记录号 | WOS:001011160500001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306215]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Zelixir Biotech, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, SSIP Healthcare & Med Demonstrat Zone, Zhongshan, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, Shanghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Meixia,Wang, Yeqing,Guo, Ciliang,et al. The claim of primacy of human gut Bacteroides ovatus in dietary cellobiose degradation[J]. GUT MICROBES,2023,15(1):18. |
| APA | Li, Meixia.,Wang, Yeqing.,Guo, Ciliang.,Wang, Sheng.,Zheng, Liangzhen.,...&Ding, Kan.(2023).The claim of primacy of human gut Bacteroides ovatus in dietary cellobiose degradation.GUT MICROBES,15(1),18. |
| MLA | Li, Meixia,et al."The claim of primacy of human gut Bacteroides ovatus in dietary cellobiose degradation".GUT MICROBES 15.1(2023):18. |
入库方式： OAI收割
来源：上海药物研究所
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