A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo
文献类型：期刊论文
| 作者 | Hu, Li2,3,4; Dong, Chanjuan5; Wang, Zhe6; He, Shengyuan5; Yang, Yiwen6; Zi, Meiting2,3; Li, Huiqin2,3; Zhang, Yanghuan1; Chen, Chuanjie4,5; Zheng, Runzi1 |
| 刊名 | AGING CELL
 |
| 出版日期 | 2023-06-13 |
| 页码 | 14 |
| ISSN号 | 1474-9718 |
| 关键词 | aging fibrosis near-infrared probe senescent cells beta-galactosidase |
| DOI | 10.1111/acel.13896 |
| 通讯作者 | Zhang, Xuan(zhangxuan@simm.ac.cn) ; He, Yonghan(heyonghan@mail.kiz.ac.cn) |
| 英文摘要 | Senescent cells (SnCs) are implicated in aging and various age-related pathologies. Targeting SnCs can treat age-related diseases and extend health span. However, precisely tracking and visualizing of SnCs is still challenging, especially in in vivo environments. Here, we developed a near-infrared (NIR) fluorescent probe (XZ1208) that targets beta-galactosidase (beta-Gal), a well-accepted biomarker for cellular senescence. XZ1208 can be cleaved rapidly by beta-Gal and produces a strong fluorescence signal in SnCs. We demonstrated the high specificity and sensitivity of XZ1208 in labeling SnCs in naturally aged, total body irradiated (TBI), and progeroid mouse models. XZ1208 achieved a long-term duration of over 6 days in labeling senescence without causing significant toxicities and accurately detected the senolytic effects of ABT263 on eliminating SnCs. Furthermore, XZ1208 was applied to monitor SnCs accumulated in fibrotic diseases and skin wound healing models. Overall, we developed a tissue-infiltrating NIR probe and demonstrated its excellent performance in labeling SnCs in aging and senescence-associated disease models, indicating great potential for application in aging studies and diagnosis of senescence-associated diseases. |
| WOS关键词 | BETA-GALACTOSIDASE ; CELLS |
| 资助项目 | Basic Research Project of Yunnan Province[202201AS070038] ; National Natural Science Foundation of China[22277131] ; National Natural Science Foundation of China[82171558] ; Pioneer Hundred Talents Program ; Shanghai Pujiang Program[21PJ1415900] ; Xingdian Talent Support Program[XDYC-QNRC-2022-0771] |
| WOS研究方向 | Cell Biology ; Geriatrics & Gerontology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:001008548600001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306355]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhang, Xuan; He, Yonghan |
| 作者单位 | 1.Kunming Univ Sci & Technol, Med Sch, Lab Mol Genet Aging & Tumor, Kunming, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, Key Lab Hlth Aging Res Yunnan Prov, Kunming, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Dev Ctr, Shanghai 202103, Peoples R China 6.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China 7.Chinese Acad Sci, Kunming Inst Zool, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Li,Dong, Chanjuan,Wang, Zhe,et al. A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo[J]. AGING CELL,2023:14. |
| APA | Hu, Li.,Dong, Chanjuan.,Wang, Zhe.,He, Shengyuan.,Yang, Yiwen.,...&He, Yonghan.(2023).A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo.AGING CELL,14. |
| MLA | Hu, Li,et al."A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo".AGING CELL (2023):14. |
入库方式： OAI收割
来源：上海药物研究所
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