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"Two Birds One Stone" Strategy for the Site-Specific Analysis of Core Fucosylation and O-GlcNAcylation

文献类型：期刊论文

作者Luo, Yawen1,2; Wang, Yuqiu3,4; Tian, Yinping2; Zhou, Hu1,3; Wen, Liuqing1,2,5
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2023-06-21
页码9
ISSN号0002-7863
DOI10.1021/jacs.3c02976
通讯作者Zhou, Hu(zhouhu@simm.ac.cn) ; Wen, Liuqing(lwen@simm.ac.cn)
英文摘要Corefucosylation and O-GlcNAcylation are thetwo most famous protein glycosylation modifications that regulatediverse physiological and pathological processes in living organisms.Here, a "two birds one stone" strategy has been describedfor the site-specific analysis of core fucosylation and O-GlcNAcylation. Taking advantage of two mutant endoglycosidases (EndoF3-D165Aand EndoCC-N180H), which efficiently and specifically recognize corefucose and O-GlcNAc, glycopeptides can be labeledusing a biantennary N-glycan probe bearing azidoand oxazoline groups. Then, a temperature-sensitive poly(N-isopropylacrylamide) polymer functionalized with dibenzocyclooctynewas introduced to facilitate the enrichment of the labeled glycopeptidesfrom the complex mixture. The captured glycopeptides can be furtherreleased enzymatically by wild-type endoglycosidases (EndoF3 and EndoCC)in a traceless manner for mass spectrometry (MS) analysis. The describedstrategy allows simultaneous profiling of core-fucosylated glycoproteomeand O-GlcNAcylated glycoproteome from one complexsample by MS technology and searching the database using differentvariable modifications.
WOS关键词BETA-N-ACETYLGLUCOSAMINIDASE ; ACUTE-PHASE PROTEINS ; CHEMOENZYMATIC SYNTHESIS ; GLYCOSYNTHASE MUTANTS ; GLYCOSYLATION ; CANCER ; FUCOSE ; GLYCOPROTEINS ; MECHANISMS ; PROTEOMICS
资助项目Lingang Laboratory[LG-QS-202206-08] ; National Natural Science Foundation of China[22007092] ; Shanghai Sailing Program[20YF1457200] ; National Key Research and Development Program[2022YFA1302900] ; Shanghai Young Excellent Academic Leader Program[20XD1424900] ; Institutional Technology Service Center of Shanghai Institute of Materia Medica ; Shanghai Municipal Science and Technology Major Project
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:001016718500001
源URL[http://119.78.100.183/handle/2S10ELR8/306358]  
专题新药研究国家重点实验室
通讯作者Zhou, Hu; Wen, Liuqing
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Media, Carbohydrate Based Drug Res Ctr, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Media, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Media, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Luo, Yawen,Wang, Yuqiu,Tian, Yinping,et al. "Two Birds One Stone" Strategy for the Site-Specific Analysis of Core Fucosylation and O-GlcNAcylation[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2023:9.
APA Luo, Yawen,Wang, Yuqiu,Tian, Yinping,Zhou, Hu,&Wen, Liuqing.(2023)."Two Birds One Stone" Strategy for the Site-Specific Analysis of Core Fucosylation and O-GlcNAcylation.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,9.
MLA Luo, Yawen,et al.""Two Birds One Stone" Strategy for the Site-Specific Analysis of Core Fucosylation and O-GlcNAcylation".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2023):9.

入库方式： OAI收割

来源：上海药物研究所

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