Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling
文献类型：期刊论文
| 作者 | Liu, Simiao1,2; Wang, Jia3; Song, Bin4; Gong, Xinqi5; Liu, Huihui1,2; Hu, Qingliang1,2; Zhang, Junhui1,2,6; Li, Qianqian1,2; Zheng, Jie4; Wang, Hongwei3 |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2023-06-29 |
| 页码 | 11 |
| ISSN号 | 0032-0781 |
| 关键词 | D3 D14 D53 Receptor complex Signal transduction Strigolactone |
| DOI | 10.1093/pcp/pcad067 |
| 通讯作者 | Wang, Hongwei(hongweiwang@tsinghua.edu.cn) ; Xu, H. Eric(eric.xu@simm.ac.cn) ; Li, Jiayang(jyli@genetics.ac.cn) ; Wang, Bing(bingwang@genetics.ac.cn) |
| 英文摘要 | Strigolactones (SLs) play fundamental roles in regulating plant architecture, which is a major factor determining crop yield. The perception and signal transduction of SLs require the formation of a complex containing the receptor DWARF14 (D14), an F-box protein D3 and a transcriptional regulator D53 in an SL-dependent manner. Structural and biochemical analyses of D14 and its orthologs DAD2 and AtD14, D3 and the complexes of ASK1-D3-AtD14 and D3(CTH)-D14 have made great contributions to understanding the mechanisms of SL perception. However, structural analyses of D53 and the D53-D3-D14 holo-complex are challenging, and the biochemical mechanism underlying the complex assembly remains poorly understood. Here, we found that apo-D53 was rather flexible and reconstituted the holo-complex containing D53, S-phase kinase-associated protein 1 (SKP1), D3 and D14 with rac-GR24. The cryo-electron microscopy (cryo-EM) structure of SKP1-D3-D14 in the presence of D53 was analyzed and superimposed on the crystal structure of ASK1-D3-AtD14 without D53. No large conformational rearrangement was observed, but a 9 & ANGS; rotation appeared between D14 and AtD14. Using hydrogen-deuterium exchange monitored by mass spectrometry, we analyzed dynamic motifs of D14, D3 and D53 in the D53-SKP1-D3-D14 complex assembly process and further identified two potential interfaces in D53 that are located in the N and D2 domains, respectively. Together, our results uncovered the dynamic conformational changes and built a model of the holo-complex D53-SKP1-D3-D14, offering valuable information for the biochemical and genetic mechanisms of SL perception and signal transduction. |
| WOS关键词 | SHOOT DEVELOPMENT ; PERCEPTION ; DWARF14 ; BINDING ; RICE ; ACTS ; ARABIDOPSIS ; DEGRADATION ; INHIBITION ; REPRESSOR |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:001025065100001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306412]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Wang, Hongwei; Xu, H. Eric; Li, Jiayang; Wang, Bing |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 3.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Ctr Plant Biol,Sch Life Sci, Beijing 100084, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Res Ctr Immunol Dis, Shanghai 201203, Peoples R China 5.Renmin Univ China, Inst Math Sci, Beijing 100872, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 8.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 9.Yazhouwan Natl Lab, Sanya 572025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Simiao,Wang, Jia,Song, Bin,et al. Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling[J]. PLANT AND CELL PHYSIOLOGY,2023:11. |
| APA | Liu, Simiao.,Wang, Jia.,Song, Bin.,Gong, Xinqi.,Liu, Huihui.,...&Wang, Bing.(2023).Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling.PLANT AND CELL PHYSIOLOGY,11. |
| MLA | Liu, Simiao,et al."Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling".PLANT AND CELL PHYSIOLOGY (2023):11. |
入库方式： OAI收割
来源：上海药物研究所
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