A novel integrated automatic strategy for amino acid composition analysis of seeds from 67 species
文献类型：期刊论文
| 作者 | Ding, Yelin1,2; Bi, Qirui2; Huang, Dongdong2; Liao, Jingmei1,2; Yang, Lin1,2; Luo, Xiaoxiao2; Yang, Peilei2; Li, Yun2; Yao, Changliang2; Wei, Wenlong2 |
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2023-11-15 |
| 卷号 | 426页码:9 |
| ISSN号 | 0308-8146 |
| 关键词 | Seeds Amino acids Phenyl isothiocyanate Automatic neutral loss filtering |
| DOI | 10.1016/j.foodchem.2023.136670 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | The composition and quantity of amino acids (AAs) in seeds are complicated due to the various origins and modifications of different species. In this study, a novel automatic neutral loss filtering (ANLF) strategy based on accurate mass searching by Python was developed to analyze the free and hydrolyzed AA-phenyl isothiocyanate (PITC) derivatives from seeds of Gymnosperm and Angiosperm phyla. Compared with traditional strategies, ANLF showed much higher accuracy in screening AA derivatives by filtering nitrogen-containing non-AA com-pounds and efficiency in processing large datasets. Meanwhile, the content phenotype of 20 proteinogenic AAs from seeds of these two families was characterized by a 35-min HPLC method combined with an automated peak -matching strategy. AA profiles of 232 batches of seeds from 67 species, consisting of 19 proteinogenic AAs, 21 modified AAs, and 77 unknown AAs, would be a good reference for their application in food and medicine. |
| WOS关键词 | METABOLISM ; PROTEINS ; HPLC |
| 资助项目 | Key Program of National Natural Science Foundation of China[82130111] ; National Natural Science Foundation of China[81803716] ; Qi-Huang Chief Scientist Project of National Administration of Traditional Chinese Medicine ; SIMM-SHUTCM Traditional Chinese Medicine Innovation[E2G809H] ; Science and Technology Major Project of Inner Mongolia[2021ZD0017] |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:001027783000001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306463]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Yelin,Bi, Qirui,Huang, Dongdong,et al. A novel integrated automatic strategy for amino acid composition analysis of seeds from 67 species[J]. FOOD CHEMISTRY,2023,426:9. |
| APA | Ding, Yelin.,Bi, Qirui.,Huang, Dongdong.,Liao, Jingmei.,Yang, Lin.,...&Guo, De-an.(2023).A novel integrated automatic strategy for amino acid composition analysis of seeds from 67 species.FOOD CHEMISTRY,426,9. |
| MLA | Ding, Yelin,et al."A novel integrated automatic strategy for amino acid composition analysis of seeds from 67 species".FOOD CHEMISTRY 426(2023):9. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。