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Spatiotemporal and global profiling of DNA-protein interactions enables discovery of low-affinity transcription factors

文献类型：期刊论文

作者Guo, An-Di1,2; Yan, Ke-Nian1,2; Hu, Hao1; Zhai, Linhui1; Hu, Teng-Fei3; Su, Haixia1; Chi, Yijia1,2; Zha, Jinyin4; Xu, Yechun1; Zhao, Dongxin1,2
刊名NATURE CHEMISTRY
出版日期2023-04-27
页码18
ISSN号1755-4330
DOI10.1038/s41557-023-01196-z
通讯作者Tan, Minjia(mjtan@simm.ac.cn) ; Chen, Xiao-Hua(xhchen@simm.ac.cn)
英文摘要Precise dissection of DNA-protein interactions is essential for elucidating the recognition basis, dynamics and gene regulation mechanism. However, global profiling of weak and dynamic DNA-protein interactions remains a long-standing challenge. Here, we establish the light-induced lysine (K) enabled crosslinking (LIKE-XL) strategy for spatiotemporal and global profiling of DNA-protein interactions. Harnessing unique abilities to capture weak and transient DNA-protein interactions, we demonstrate that LIKE-XL enables the discovery of low-affinity transcription-factor/DNA interactions via sequence-specific DNA baits, determining the binding sites for transcription factors that have been previously unknown. More importantly, we successfully decipher the dynamics of the transcription factor subproteome in response to drug treatment in a time-resolved manner, and find downstream target transcription factors from drug perturbations, providing insight into their dynamic transcriptional networks. The LIKE-XL strategy offers a complementary method to expand the DNA-protein profiling toolbox and map accurate DNA-protein interactomes that were previously inaccessible via non-covalent strategies, for better understanding of protein function in health and disease.
WOS关键词CROSS-LINKING ; IDENTIFICATION ; SPECIFICITY ; CONJUGATION ; GENES
资助项目National Science Foundation of China[92053106] ; National Science Foundation of China[22225702] ; National Science Foundation of China[92153302] ; National Science Foundation of China[22177120] ; National Science Foundation of China[81821005] ; National Science Foundation of China[21907100] ; National Key Science & Technology Program of China[2020YFE0202200] ; Program of Shanghai Academic Research Leader[22XD1420900] ; Innovative Research Team of High-Level Local Universities in Shanghai[SHSMU-ZDCX20212700] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Guangdong High-Level Innovative Research Institute[2021B0909050003]
WOS研究方向Chemistry
语种英语
出版者NATURE PORTFOLIO
WOS记录号WOS:000979886400003
源URL[http://119.78.100.183/handle/2S10ELR8/306487]  
专题新药研究国家重点实验室
通讯作者Tan, Minjia; Chen, Xiao-Hua
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China
4.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Natl Res Ctr Translat Med Shanghai,State Key Lab M, Shanghai, Peoples R China
5.Jiangnan Univ, Sch Food Sci & Technol, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan, Peoples R China
7.Jiangsu Ocean Univ, Coll Pharm, Lianyungang, Peoples R China
8.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Guo, An-Di,Yan, Ke-Nian,Hu, Hao,et al. Spatiotemporal and global profiling of DNA-protein interactions enables discovery of low-affinity transcription factors[J]. NATURE CHEMISTRY,2023:18.
APA Guo, An-Di.,Yan, Ke-Nian.,Hu, Hao.,Zhai, Linhui.,Hu, Teng-Fei.,...&Chen, Xiao-Hua.(2023).Spatiotemporal and global profiling of DNA-protein interactions enables discovery of low-affinity transcription factors.NATURE CHEMISTRY,18.
MLA Guo, An-Di,et al."Spatiotemporal and global profiling of DNA-protein interactions enables discovery of low-affinity transcription factors".NATURE CHEMISTRY (2023):18.

入库方式： OAI收割

来源：上海药物研究所

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