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Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing

文献类型：期刊论文

作者Tian Jr, He3,4; Guo, Chen3,4; Hu, Xi-Le3,4; Wang, Jing-Bo3,4; Zang, Yi2,6; James, Tony D. D.1,7; Li, Jia6; He, Xiao-Peng3,4,5
刊名ORGANIC & BIOMOLECULAR CHEMISTRY
出版日期2023-06-07
卷号21期号:22页码:4661-4666
ISSN号1477-0520
DOI10.1039/d3ob00637a
通讯作者Zang, Yi(yzang@simm.ac.cn) ; James, Tony D. D.(t.d.james@bath.ac.uk) ; Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要Peroxynitrite (ONOO-) is an important oxygen/nitrogen reactive species implicated in a number of physiological and pathological processes. However, due to the complexity of the cellular micro-environment, the sensitive and accurate detection of ONOO- remains a challenging task. Here, we developed a long-wavelength fluorescent probe based on the conjugation between a TCF scaffold and phenylboronate; the resulting conjugate is capable of supramolecular host-guest assembly with human serum albumin (HSA) for the fluorogenic sensing of ONOO-. The probe exhibited an enhanced fluorescence over a low concentration range of ONOO- (0-9.6 mu M), whist the fluorescence was quenched when the concentration of ONOO- exceeded 9.6 mu M. In addition, when human serum albumin (HSA) was added, the initial fluorescence of the probe was significantly enhanced, which enabled the more sensitive detection of low-concentrations of ONOO- in aqueous buffer solution and in cells. The molecular structure of the supramolecular host-guest ensemble was determined using small-angle X-ray scattering.
WOS关键词FLUORESCENT-PROBE ; DISEASES ; CELLS ; HSA
资助项目National Natural Science Foundation of China (NSFC)[92253306] ; National Natural Science Foundation of China (NSFC)[82130099] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; Fundamental Research Funds for the Central Universities[222201717003] ; Programme of Introducing Talents of Discipline to Universities[B16017] ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology ; Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University[2020ZD01]
WOS研究方向Chemistry
语种英语
出版者ROYAL SOC CHEMISTRY
WOS记录号WOS:000991907300001
源URL[http://119.78.100.183/handle/2S10ELR8/306502]  
专题新药研究国家重点实验室
通讯作者Zang, Yi; James, Tony D. D.; Li, Jia; He, Xiao-Peng
作者单位1.Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China
2.Lingang Lab, Shanghai 201203, Peoples R China
3.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China
4.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China
5.Int Cooperat Lab Signal Transduct, Natl Ctr Liver Canc, Shanghai 200438, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China
7.Univ Bath, Dept Chem, Bath BA2 7AY, England
推荐引用方式
GB/T 7714		 Tian Jr, He,Guo, Chen,Hu, Xi-Le,et al. Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2023,21(22):4661-4666.
APA Tian Jr, He.,Guo, Chen.,Hu, Xi-Le.,Wang, Jing-Bo.,Zang, Yi.,...&He, Xiao-Peng.(2023).Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing.ORGANIC & BIOMOLECULAR CHEMISTRY,21(22),4661-4666.
MLA Tian Jr, He,et al."Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing".ORGANIC & BIOMOLECULAR CHEMISTRY 21.22(2023):4661-4666.

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来源：上海药物研究所

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