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A pseudotargeted peptidomics strategy for screening natural signature peptides in animal- derived drugs: Taking Pheretima as a case

文献类型：期刊论文

作者Huang, Dongdong2,3; Luo, Xiaoxiao1,2; Bi, Qirui2; Ding, Yelin2,4; Li, Yun2; Wang, Cuicui2; Gao, Min2; Huang, Yong2; Yao, Changliang2; Zhang, Jianqing2
刊名ARABIAN JOURNAL OF CHEMISTRY
出版日期2023-08-01
卷号16期号:8页码:10
关键词Natural peptides Pseudotargeted peptidomics Signature peptides Animal-derived drugs Pheretima
ISSN号1878-5352
DOI10.1016/j.arabjc.2023.104980
通讯作者Wang, Yurong(1620154332@cpu.edu.cn) ; Guo, De-an(daguo@simm.ac.cn)
英文摘要Natural peptides (NPs) are ubiquitous throughout the organism with diversified struc-tures and wide dynamic concentration range, which leads to serious challenge for their comprehen-sive analysis. Herein, a pseudotargeted peptidomics strategy based on dynamic multiple reaction monitoring (DMRM) with high-throughput data acquisition and accurate quantification was estab-lished. Specifically, an in-house software Pep-MRMer was developed for extraction of ions infor-mation of peptides, followed by de-redundancy and fusion to construct a transition list. Additionally, retention time (RT) calibration method was optimized by using high content compo-nents for effective transference of ion pairs between different detection conditions (e.g., instruments, chromatographic gradients). This strategy was applied for profiling NPs and screening signature peptides for species authentication of Pheretima, a multi-source animal-derived drug widely used in Asian countries. In all, a total of 3307 peptides had excellent quantitative detection after trans-ference. Subsequently, nine signature peptides with extraordinary specificity were screened, identi- fied, and confirmed by comparing with the synthetic standards. Ultimately, a 10 min specific chro-matogram based on signature peptides was developed, which realized rapid and accurate identifica-tion of Pheretima. Taken together, this research provides a robust strategy for species discrimination of animal-derived drugs without adequate quality control measures.(c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
WOS关键词METABOLOMICS METHOD ; SPECTROMETRY ; PLASMA
资助项目Key Program of National Natural Science Foundation of China[82130111] ; National Natural Science Foundation of China[81803716] ; Qi-Huang Chief Scientist Project of National Administration of Traditional Chinese Medicine
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001003453500001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/306686]  
专题中国科学院上海药物研究所
通讯作者Wang, Yurong; Guo, De-an
作者单位1.China Pharmaceut Univ, Nanjing 211198, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
4.Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China
5.Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
6.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Dongdong,Luo, Xiaoxiao,Bi, Qirui,et al. A pseudotargeted peptidomics strategy for screening natural signature peptides in animal- derived drugs: Taking Pheretima as a case[J]. ARABIAN JOURNAL OF CHEMISTRY,2023,16(8):10.
APA Huang, Dongdong.,Luo, Xiaoxiao.,Bi, Qirui.,Ding, Yelin.,Li, Yun.,...&Guo, De-an.(2023).A pseudotargeted peptidomics strategy for screening natural signature peptides in animal- derived drugs: Taking Pheretima as a case.ARABIAN JOURNAL OF CHEMISTRY,16(8),10.
MLA Huang, Dongdong,et al."A pseudotargeted peptidomics strategy for screening natural signature peptides in animal- derived drugs: Taking Pheretima as a case".ARABIAN JOURNAL OF CHEMISTRY 16.8(2023):10.

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来源：上海药物研究所

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