Precolumn derivatization LC-MS/MS method to simultaneous quantitation of 10 monosaccharides in rat plasma
文献类型：期刊论文
| 作者 | Li, Mengxue1,2; Li, Ping2; Ji, Yuting2; Tian, Yihua2; Zeng, Huiying2; Chen, Xiaoyan2,3
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 出版日期 | 2023-09-20 |
| 卷号 | 234页码:10 |
| 关键词 | LC-MS MS Monosaccharides Derivatization 1-Phenyl-3-methyl-5-pyrazolone (PMP) Biomarker |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2023.115537 |
| 通讯作者 | Chen, Xiaoyan(xychen@simm.ac.cn) |
| 英文摘要 | Monosaccharides are essential for maintaining the normal physiological functions of living organisms. Under disease states, metabolic disorders in vivo will inevitably affect the levels of monosaccharides, which brings the possibility of monosaccharides as a biomarker of some diseases. In this study, a method was developed and validated for simultaneously determining 10 monosaccharides (glucose, galactose, mannose, rhamnose, fucose, xylose, iduronic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine) in SD rat plasma using liquid chromatography-tandem mass spectrometry. The method employed 1-phenyl-3-methyl-5-pyrazolone (PMP) as a derivatization reagent, considerably improved the chromatographic retention and ionization efficiency of monosaccharides. After protein precipitation of plasma samples, monosaccharides and isotope internal standards were derivatized and liquid-liquid extraction was performed to remove excess PMP. To achieve the baseline separation of several isomers, the resulting derivatives were chromatographed on a Bridged ethyl hybrid (BEH) Phenyl column using gradient elution with a total run time of 8 min. The method was linear within the range of 0.0100-5.00 & mu;g/mL for rhamnose, 0.0500-25.0 & mu;g/mL for fucose, xylose, iduronic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine, 1.00-500 & mu;g/mL for galactose, 10.0-5000 & mu;g/mL for mannose, and 50.0-25,000 & mu;g/mL for glucose. And the accuracy and precision verification of surrogate matrix samples and plasma samples met the required criteria. The method has been used successfully to study the effect of hepatic insufficiency on monosaccharide levels in rats. It was found that the concentration of glucuronic acid in SD rat plasma was abnormally increased in rats with liver injury. |
| WOS关键词 | LIQUID-CHROMATOGRAPHY ; FUCOSE ; OLIGOSACCHARIDES ; POLYSACCHARIDES ; BIOSYNTHESIS ; CANCER ; SUGARS ; LEVEL ; SERUM ; ACID |
| 资助项目 | High-level New Ramp;D Institute[2019B090904008] ; High-level Innovative Research Institute[2021B0909050003] ; Department of Science and Technology of Guangdong Province |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001034127800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306725]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Zunyi Med Univ, Sch Pharm, 1 Campus Rd, Zunyi 563000, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528437, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Mengxue,Li, Ping,Ji, Yuting,et al. Precolumn derivatization LC-MS/MS method to simultaneous quantitation of 10 monosaccharides in rat plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2023,234:10. |
| APA | Li, Mengxue,Li, Ping,Ji, Yuting,Tian, Yihua,Zeng, Huiying,&Chen, Xiaoyan.(2023).Precolumn derivatization LC-MS/MS method to simultaneous quantitation of 10 monosaccharides in rat plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,234,10. |
| MLA | Li, Mengxue,et al."Precolumn derivatization LC-MS/MS method to simultaneous quantitation of 10 monosaccharides in rat plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 234(2023):10. |
入库方式： OAI收割
来源：上海药物研究所
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