Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration
文献类型：期刊论文
| 作者 | Wei, Wei1,2; Wang, Lian-Yan1; Yuan, Lan3; Yang, Xiao-Da3; Su, Zhi-Guo1; Ma, Guang-Hui1 |
| 刊名 | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 69期号:3页码:878-886 |
| 关键词 | chitosan microsphere bioadhesion absorption distribution biodegradation |
| ISSN号 | 0935-1221 |
| 其他题名 | Eur. J. Pharm. Biopharm. |
| 中文摘要 | Chitosan microspheres have a great potential in pharmaceutical application. In this study, uniform-sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) were prepared by Shirasu Porous Glass (SPG) membrane emulsification technique. Based on the characterizations of uniform size and autofluorescence, it was possible to develop a new detecting system for observing and quantifying the CG microspheres in rats with three different diameters (2.1. 7.2 and 12.5 mu m) synchronously after oral administration. This system was a combination of scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and flow cytometer technique. which showed the advantages of being simple, intuitionistic. repeatable and sensitive. After oral administration of three kinds of particles with different diameters. bioadhesion in gastrointestinal tract, absorption in gastrointestinal tract, distribution in systemic tissues, and biodegradation in reticuloendothelial system (RES) were studied firstly in detail. The CG microspheres showed different fates in bioadhesion, absorption and distribution according to their diameters, while the biodegradation also varied due to the different locations in RES. These original results would indicate a better way for the CG microspheres in the clinical application. (C) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | Chitosan microspheres have a great potential in pharmaceutical application. In this study, uniform-sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) were prepared by Shirasu Porous Glass (SPG) membrane emulsification technique. Based on the characterizations of uniform size and autofluorescence, it was possible to develop a new detecting system for observing and quantifying the CG microspheres in rats with three different diameters (2.1. 7.2 and 12.5 mu m) synchronously after oral administration. This system was a combination of scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and flow cytometer technique. which showed the advantages of being simple, intuitionistic. repeatable and sensitive. After oral administration of three kinds of particles with different diameters. bioadhesion in gastrointestinal tract, absorption in gastrointestinal tract, distribution in systemic tissues, and biodegradation in reticuloendothelial system (RES) were studied firstly in detail. The CG microspheres showed different fates in bioadhesion, absorption and distribution according to their diameters, while the biodegradation also varied due to the different locations in RES. These original results would indicate a better way for the CG microspheres in the clinical application. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | DELIVERY SYSTEMS ; NANOPARTICLES ; NANOSPHERES ; HEMOGLOBIN ; PROTEINS ; CAPACITY ; RELEASE ; DRUG |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000258882200010 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2883]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Dept Biol Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wei,Wang, Lian-Yan,Yuan, Lan,et al. Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2008,69(3):878-886. |
| APA | Wei, Wei,Wang, Lian-Yan,Yuan, Lan,Yang, Xiao-Da,Su, Zhi-Guo,&Ma, Guang-Hui.(2008).Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,69(3),878-886. |
| MLA | Wei, Wei,et al."Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 69.3(2008):878-886. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。