Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells
文献类型：期刊论文
| 作者 | Gong, Daohua1,3,5; Wang, Lin1,5; Zhou, Hu2 ; Gao, Jing2; Zhang, Weidao1,5; Zheng, Ping1,4,5
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| 刊名 | STEM CELL REPORTS
 |
| 出版日期 | 2023-04-11 |
| 卷号 | 18期号:4页码:952-968 |
| ISSN号 | 2213-6711 |
| DOI | 10.1016/j.stemcr.2023.02.003 |
| 通讯作者 | Zheng, Ping(zhengp@mail.kiz.ac.cn) |
| 英文摘要 | Embryonic stem cells (ESCs) are superior to differentiated cells to maintain genome stability, but the underlying mechanisms remain largely elusive. R-loops are constantly formed during transcription and are inducers of DNA damage if not resolved. Here we report that mouse ESCs (mESCs) can efficiently prevent unscheduled R-loop formation, and a long noncoding RNA Lnc530 plays regulatory role. Lnc530 is expressed in mESCs and localizes on R-loops. Depletion of Lnc530 in mESCs causes R-loop accumulation and DNA damage, whereas forced expression of Lnc530 in differentiated cells suppresses the R-loop formation. Mechanistically, Lnc530 associates with DDX5 and TDP-43 in an inter-dependent manner on R-loops. Formation of Lnc530-DDX5-TDP-43 complex substantially increases the local protein levels of DDX5 and TDP-43, both of which play critical roles in R-loop regulation. This study uncovers an efficient strategy to prevent R-loop accumulation and preserve genomic stability in mESCs and possibly other stem cell types. |
| WOS关键词 | DNA-DAMAGE RESPONSE ; TRANSCRIPTION ; REPLICATION ; HYBRIDS ; REPAIR ; TDP-43 ; INTEGRITY ; PREVALENT ; THREATS ; FILIA |
| 资助项目 | Chinese Acad-emy of Sciences ; National Natural Science Foundation of China[31930027] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:001033008700001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306779]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zheng, Ping |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650203, Yunnan, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 4.Chinese Acad Sci, Kunming Inst Zool, CUHK Joint Lab Bioresources & Mol Res Common Dis, KIZ, Kunming 650203, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech Yunnan Prov, Kunming 650203, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Daohua,Wang, Lin,Zhou, Hu,et al. Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells[J]. STEM CELL REPORTS,2023,18(4):952-968. |
| APA | Gong, Daohua,Wang, Lin,Zhou, Hu,Gao, Jing,Zhang, Weidao,&Zheng, Ping.(2023).Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells.STEM CELL REPORTS,18(4),952-968. |
| MLA | Gong, Daohua,et al."Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells".STEM CELL REPORTS 18.4(2023):952-968. |
入库方式： OAI收割
来源：上海药物研究所
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