A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits A beta(42) production via activating autophagy
文献类型：期刊论文
| 作者 | Li, Saijuan1,2; Wu, Fangge1,2; Gao, Pengcheng1,2; Jin, Can2,3,4; Wang, Yuyong1,2; Liao, Wenfeng2,3; Ding, Kan1,2,3,4
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| 刊名 | FITOTERAPIA
 |
| 出版日期 | 2023-09-01 |
| 卷号 | 169页码:11 |
| 关键词 | Semiaquilegia adoxoides Amyloid beta Autophagy Peptidoglycan Alzheimer's disease |
| ISSN号 | 0367-326X |
| DOI | 10.1016/j.fitote.2023.105552 |
| 通讯作者 | Liao, Wenfeng(liaowenfeng@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | The accumulation of amyloid beta (A beta) containing senile plaques is one of the key histopathological hallmarks of Alzheimer's disease (AD). Increasing evidences demonstrated the important role of autophagy in A beta clearance. Recent studies implied that extracts from Semiaquilegia adoxoides (DC.) Makino could ameliorate the memory of D-galactose induced aging mice. However, the bioactive substance and underlying mechanism remains unknown. Thus, the present study sought to explore the effects of a novel homogenous peptidoglycan on A beta(42) secretion and the underlying mechanism. Briefly, we extracted a novel peptidoglycan named SA02C using hot water extraction and alcohol precipitation with the Mw of 13.72 kDa. SA02C contains 73.33% carbohydrate and 27.83% protein. The structure characterization revealed that its glycan part might mainly composed of galacturonic acid with minor rhamnose in backbone, and branched with glucose, galactose, arabinose, xylose and galacturonic acid. The protein or peptide moiety in SA02C was bonded to the polysaccharide via threonine. Bioactivities test showed that SA02C could reduce A beta(42) production in a dose dependent manner with no obvious cytotoxicity. Mechanism study demonstrated that SA02C could modulate APP processing by upregulating the expression of ADAM10, sAPPa and downregulating BACE1, sAPP beta. Furthermore, SA02C also could stimulate autophagy by promoting the expression of the markers of autophagy such as LC3B and ATG5, resulting in the promotion of A beta(42) phagocytosis. |
| WOS关键词 | ALZHEIMERS-DISEASE ; HEXENURONIC ACID ; MOUSE MODEL ; BETA ; PECTIN ; POLYSACCHARIDE ; PROTEOGLYCAN ; EXTRACT ; ALTERS ; FRUITS |
| 资助项目 | National Natural Science Foundation of China[3187081] ; Shanghai Municipal Science and Technology Major Project[LG202101-01-02] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001026403800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306794]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Liao, Wenfeng; Ding, Kan |
| 作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Carbohydrate Based Drug Res Ctr,CAS Key Lab Recept, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528400, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Saijuan,Wu, Fangge,Gao, Pengcheng,et al. A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits A beta(42) production via activating autophagy[J]. FITOTERAPIA,2023,169:11. |
| APA | Li, Saijuan.,Wu, Fangge.,Gao, Pengcheng.,Jin, Can.,Wang, Yuyong.,...&Ding, Kan.(2023).A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits A beta(42) production via activating autophagy.FITOTERAPIA,169,11. |
| MLA | Li, Saijuan,et al."A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits A beta(42) production via activating autophagy".FITOTERAPIA 169(2023):11. |
入库方式： OAI收割
来源：上海药物研究所
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