Integrative Profiling of Lysine Acylome in Sepsis-Induced Liver Injury
文献类型：期刊论文
| 作者 | Na, Ann-Yae1; Choi, So Young2; Paudel, Sanjita3; Bae, Jong-Sup3; Tan, Minjia4 ; Lee, Sangkyu1,5
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2023-07-31 |
| 页码 | 11 |
| ISSN号 | 1535-3893 |
| 关键词 | cecum ligation and puncture model sepsis lysine acylation liver dysfunction protein modification |
| DOI | 10.1021/acs.jproteome.3c00130 |
| 通讯作者 | Lee, Sangkyu(sangkyu@skku.edu) |
| 英文摘要 | Sepsis is one of the life-threatening diseases worldwide.Despitethe continuous progress in medicine, the specific mechanism of sepsisremains unclear. A key strategy of pathogens is to use post-translationalmodification to modulate host factors critical for infection. We employedglobal immunoprecipitation technology for lysine acetylation (Kac),succinylation (Ksu), and malonylation (Kmal) for the first globallysine acylation (Kacy) analysis in a cecum ligation and puncture(CLP) model in mouse. This was performed to reveal the pathogenicmechanism of integrative Kacy and the changes in modification sites.In total, 2230 sites of 1,235 Kac proteins, 1,887 sites of 433 Ksuproteins, and 499 sites of 276 Kmal proteins were quantified and normalizedby their protein levels. We focused on 379 sites in 219 upregulatedproteins as the integrative Kacy sites of Kac, Ksu, and Kmal on thebasis of sirtuins decreased in the CLP group. KEGG pathway analysisof integrative Kacy in 219 upregulated proteins revealed three centralmetabolic pathways: glycolysis/gluconeogenesis, pyruvate metabolism,and tricarboxylic acid cycle. These findings reveal the key pathogenicmechanism of integrative PTM alteration in terms of the decreasedsirtuins level and provide an important foundation for an in-depthstudy of the biological function of Kacy in sepsis. |
| WOS关键词 | METABOLISM ; SUCCINYLATION ; MALONYLATION ; SIRT5 |
| 资助项目 | National Research Foundation of Korea (NRF) - Korea government (MSIP)[2021R1A2C1003510] ; National Research Foundation of Korea (NRF) - Korea government (MSIP)[2021R1A6A3A01086425] ; National Research Foundation of Korea (NRF) - Korea government (MSIP)[2021K2A9A2A06044011] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:001038281000001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306806]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Lee, Sangkyu |
| 作者单位 | 1.Sungkyunkwan Univ, Global Drug Dev Res Inst, Suwon 16419, South Korea 2.Kyungpook Natl Univ, Mass Spectrometry Convergence Res Ctr, Daegu 41566, South Korea 3.Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 5.Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea |
| 推荐引用方式 GB/T 7714 | Na, Ann-Yae,Choi, So Young,Paudel, Sanjita,et al. Integrative Profiling of Lysine Acylome in Sepsis-Induced Liver Injury[J]. JOURNAL OF PROTEOME RESEARCH,2023:11. |
| APA | Na, Ann-Yae,Choi, So Young,Paudel, Sanjita,Bae, Jong-Sup,Tan, Minjia,&Lee, Sangkyu.(2023).Integrative Profiling of Lysine Acylome in Sepsis-Induced Liver Injury.JOURNAL OF PROTEOME RESEARCH,11. |
| MLA | Na, Ann-Yae,et al."Integrative Profiling of Lysine Acylome in Sepsis-Induced Liver Injury".JOURNAL OF PROTEOME RESEARCH (2023):11. |
入库方式： OAI收割
来源：上海药物研究所
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