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Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered & alpha;-Hemolysin Nanopores

文献类型：期刊论文

作者Xia, Bingqing2,3; Fang, Jie1,2,3; Ma, Shengzhou3,4; Ma, Mengyao2,3; Yao, Guangda2,3,5; Li, Tiehai2,3; Cheng, Xi2,3,6; Wen, Liuqing3,4; Gao, Zhaobing1,2,3
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2023-08-01
页码13
ISSN号0002-7863
DOI10.1021/jacs.3c03563
通讯作者Xia, Bingqing(xiabingqing@simm.ac.cn) ; Cheng, Xi(xicheng@simm.ac.cn) ; Wen, Liuqing(lwen@simm.ac.cn) ; Gao, Zhaobing(zbgao@simm.ac.cn)
英文摘要Glycan is a crucial class of biological macromoleculeswith importantbiological functions. Functional groups determine the chemical propertiesof glycans, which further affect their biological activities. However,the structural complexity of glycans has set a technical hurdle fortheir direct identification. Nanopores have emerged as highly sensitivebiosensors that are capable of detecting and characterizing variousanalytes. Here, we identified the functional groups on glycans witha designed & alpha;-hemolysin nanopore containing arginine mutations(M113R), which is specifically sensitive to glycans with acetamidoand carboxyl groups. Molecular dynamics simulations indicated thatthe acetamido and carboxyl groups of the glycans produce unique electricalsignatures by forming polar and electrostatic interactions with theM113R nanopores. Using these electrical features as the fingerprints,we mapped the length of the glycans containing acetamido and carboxylgroups at the monosaccharide, disaccharide, and trisaccharide levels.This proof-of-concept study provides a promising foundation for developingsingle-molecule glycan fingerprinting libraries and demonstrates thecapability of biological nanopores in glycan sequencing.
WOS关键词ALPHA-HEMOLYSIN ; STOCHASTIC DETECTION ; ELECTROPHORESIS ; CYCLODEXTRIN ; DYNAMICS ; ADAPTER
资助项目National Science Fund of Distinguished Young Scholars[81825021] ; Fund of Youth Innovation Promotion Association[2019285] ; Fund of Youth Innovation Promotion Association[2021333] ; Fund of Youth Innovation Promotion Association[2022077] ; National Natural Science Foundation of China[81773707] ; National Natural Science Foundation of China[31700732] ; Shanghai Municipal Science and Technology Major Project ; Fund of Shanghai Science and Technology Innovation Action Plan[20ZR1474200] ; Shanghai Rising-Star Program[22QA1411000] ; Natural Science Foundation of Shanghai[22ZR1474000] ; National Key Laboratory Program of China[LG202101-01-04] ; National Key Laboratory Program of China[LG202102-01-01] ; National Key Research and Development Program of China[2020YFC0842000] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences[XDA12050308]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001040987100001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/306830]  
专题新药研究国家重点实验室
通讯作者Xia, Bingqing; Cheng, Xi; Wen, Liuqing; Gao, Zhaobing
作者单位1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210046, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, Shanghai 201203, Peoples R China
5.Tech Univ, Sch Life Sci & Technol, Lingang Lab, Shanghai 200031, Peoples R China
6.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Xia, Bingqing,Fang, Jie,Ma, Shengzhou,et al. Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered & alpha;-Hemolysin Nanopores[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2023:13.
APA Xia, Bingqing.,Fang, Jie.,Ma, Shengzhou.,Ma, Mengyao.,Yao, Guangda.,...&Gao, Zhaobing.(2023).Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered & alpha;-Hemolysin Nanopores.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,13.
MLA Xia, Bingqing,et al."Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered & alpha;-Hemolysin Nanopores".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2023):13.

入库方式： OAI收割

来源：上海药物研究所

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