Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19
文献类型：期刊论文
| 作者 | Xu, Haiyu1; Li, Shufen2; Liu, Jiayuan3; Cheng, Jinlong4; Kang, Liping1; Li, Weijie1; Zhong, Yute1; Wei, Chaofa; Fu, Lifeng4; Qi, Jianxun4,6 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2023-04-25 |
| 卷号 | 120期号:18页码:11 |
| ISSN号 | 0027-8424 |
| 关键词 | COVID-19 traditional Chinese medicine TCMT-NDRD antivirus anti-inflammation |
| DOI | 10.1073/pnas.2301775120 |
| 通讯作者 | Gao, George F.(gaof@im.ac.cn) ; Huang, Luqi(huangluqi01@126.com) |
| 英文摘要 | The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global health concern, and effective antiviral reagents are urgently needed. Traditional Chinese medicine theory -driven natural drug research and development (TCMT-NDRD) is a feasible method to address this issue as the traditional Chinese medicine formulae have been shown effective in the treatment of COVID-19. Huashi Baidu decoction (Q-14) is a clinically approved formula for COVID-19 therapy with antiviral and anti-inflammatory effects. Here, an integrative pharmacological strategy was applied to identify the antiviral and anti-inflammatory bioactive compounds from Q-14. Overall, a total of 343 chemical compounds were initially characterized, and 60 prototype compounds in Q-14 were subsequently traced in plasma using ultrahigh -performance liquid chromatography with quadrupole time -of -flight mass spectrometry. Among the 60 compounds, six compounds (magnolol, glycyrrhisoflavone, licoisoflavone A, emodin, echinatin, and quercetin) were identified showing a dose -dependent inhibition effect on the SARS-CoV-2 infection, including two inhibitors (echinatin and quercetin) of the main protease (Mpro), as well as two inhibitors (glycyrrhisoflavone and licoisoflavone A) of the RNA -dependent RNA polymerase (RdRp). Meanwhile, three anti-inflammatory components, including licochalcone B, echinatin, and glycyrrhisoflavone, were identified in a SARS-CoV-2-infected inflammatory cell model. In addition, glycyrrhisoflavone and licoisoflavone A also displayed strong inhibitory activities against cAMP-specific 3 & PRIME;,5 & PRIME;-cyclic phosphodiesterase 4 (PDE4). Crystal structures of PDE4 in complex with glycyrrhisoflavone or licoisoflavone A were determined at resolutions of 1.54 & ANGS; and 1.65 & ANGS;, respectively, and both compounds bind in the active site of PDE4 with similar interactions. These findings will greatly stimulate the study of TCMT-NDRD against COVID-19. |
| WOS关键词 | MODEL ; CORONAVIRUS ; ARTEMISININ ; SOFTWARE ; FORMULA ; DNA |
| 资助项目 | Key Project at the Central Government Level: The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources[2060302] ; National Natural Science Foundation of China[81830111] ; Establishment of Sino-Austria Belt and Road Joint Laboratory on Traditional Chinese Medicine for Severe Infectious Diseases and Joint Research[2020YFE0205100] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:001038055100001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306893]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Gao, George F.; Huang, Luqi |
| 作者单位 | 1.Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Antiviral Res, State Key Lab Virol, Wuhan 430207, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Chinese Acad Sci CAS, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Daodi Herbs, Beijing 100700, Peoples R China 6.Beijing Life Sci Acad, Beijing 102209, Peoples R China 7.Chinese Acad Med Sci & Peking Union Med Coll, Inst Lab Anim Sci, Beijing Key Lab Anim Models Emerging & Reemerging, Key Lab Comparat Med Human Dis,Natl Hlth Commiss, Beijing 100021, Peoples R China 8.Tsinghua Univ, Inst Tradit Chinese Med X, Beijing Natl Res Ctr Informat Sci & Technol, Dept Automat,Minist Educ,Bioinformat Div,Key Lab B, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Haiyu,Li, Shufen,Liu, Jiayuan,et al. Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2023,120(18):11. |
| APA | Xu, Haiyu.,Li, Shufen.,Liu, Jiayuan.,Cheng, Jinlong.,Kang, Liping.,...&Huang, Luqi.(2023).Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,120(18),11. |
| MLA | Xu, Haiyu,et al."Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 120.18(2023):11. |
入库方式： OAI收割
来源：上海药物研究所
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