Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics
文献类型：期刊论文
| 作者 | Ma, Wenjing1,2; Xu, Zhuojia1,2; Jiang, Yuhan1; Liu, Jialin1; Xu, Dandan3; Huang, Wei1,3; Li, Tiehai1,2 |
| 刊名 | ADVANCED SCIENCE
 |
| 出版日期 | 2023-08-26 |
| 页码 | 10 |
| 关键词 | carbohydrates enzyme-catalyzed synthesis functional glycomics glycan microarray IgG N-glycan library |
| DOI | 10.1002/advs.202303832 |
| 通讯作者 | Li, Tiehai(tiehaili@simm.ac.cn) |
| 英文摘要 | N-Glycosylation, a main post-translational modification of Immunoglobulin G (IgG), plays a significant role in modulating the immune functions of IgG. However, the precise function elucidation of IgG N-glycosylation remains impeded due to the obstacles in obtaining comprehensive and well-defined N-glycans. Here, an easy-to-implement divergent approach is described to synthesize a 64-membered IgG N-glycan library covering all possible biantennary and bisected N-glycans by reprogramming biosynthetic assembly lines based on the inherent branch selectivity and substrate specificity of enzymes. The unique binding specificities of 64 N-glycans with different proteins are deciphered by glycan microarray technology. This unprecedented collection of synthetic IgG N-glycans can serve as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics to facilitate biomedical applications. |
| WOS关键词 | CHEMOENZYMATIC SYNTHESIS ; SIALYLGLYCOPEPTIDE SGP ; BRANCH SPECIFICITY ; SCALE PRODUCTION ; GLYCOSYLATION ; OLIGOSACCHARIDES ; TRIANTENNARY ; BIANTENNARY ; STRATEGY ; BI |
| 资助项目 | W.M. and Z.X. contributed equally to this work. This work was financially supported by the National Natural Science Foundation of China (22077130), the Science and Technology Commission of Shanghai Municipality (20ZR1467900), the CAS Pioneer Talent Program[20ZR1467900] ; National Natural Science Foundation of China ; Science and Technology Commission of Shanghai Municipality ; CAS Pioneer Talent Program ; Shanghai Municipal Science and Technology Major Project ; [22077130] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:001054595200001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307025]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Tiehai |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Wenjing,Xu, Zhuojia,Jiang, Yuhan,et al. Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics[J]. ADVANCED SCIENCE,2023:10. |
| APA | Ma, Wenjing.,Xu, Zhuojia.,Jiang, Yuhan.,Liu, Jialin.,Xu, Dandan.,...&Li, Tiehai.(2023).Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.ADVANCED SCIENCE,10. |
| MLA | Ma, Wenjing,et al."Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics".ADVANCED SCIENCE (2023):10. |
入库方式： OAI收割
来源：上海药物研究所
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