An inulin-type fructan isolated from Serratula chinensis alleviated the dextran sulfate sodium-induced colitis in mice through regulation of intestinal barrier and gut microbiota
文献类型：期刊论文
| 作者 | Li, Zhenwei1; Wen, Qiuyi1,2; Pi, Jiaju1; Zhang, Daidi1; Nie, Jinchun1; Wei, Wenlong3; Li, Wei1; Guo, De-an1,3,4
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2023-11-15 |
| 卷号 | 320页码:12 |
| ISSN号 | 0144-8617 |
| 关键词 | Polysaccharide Fructan Serratula chinensis Colitis Intestinal barrier Metagenomic sequencing |
| DOI | 10.1016/j.carbpol.2023.121206 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | Herein, we aimed to explore the polysaccharide material basis of Serratula chinensis and establish its beneficial effects against colitis. A neutral polysaccharide (SCP) was extracted from S. chinensis in high yield using hot water. The molecular weights were calculated by HPSEC as Mw = 2928 Da, Mn = 2634 Da, and Mw/Mn = 1.11. FT-IR and 1D/2D-NMR spectroscopic analyses confirmed that SCP was an inulin-type fructan with & alpha;-D-Glcp-(1 & RARR; [1)-& beta;-D-Fruf-(2]17) linkages. Treatment with SCP (200 or 400 mg/kg) alleviated dextran sulfate sodium (DSS)induced mouse colitis symptoms, including the loss of body weight, increase of disease activity index score, and shortening of colon length. Histopathological and immunofluorescence assessments revealed that SCP could reduce pathological damage to the colon, restore the number of goblet cells, increase the content of glycoproteins in goblet cells and mucins in crypts, and enhance the expression of tight junction proteins ZO-1 and occludin. In addition, metagenomic sequencing revealed that SCP could improve the dysbiosis of gut microbiomes and act on multiple microbial functions. Moreover, SCP treatment increased the content of colonic acetic acid and butanoic acid. Collectively, these results indicated that SCP could alleviate the DSS-induced colitis in mice through regulation of intestinal barrier and gut microbiota. |
| WOS关键词 | STRUCTURAL-CHARACTERIZATION ; ULCERATIVE-COLITIS ; ARCTIUM-LAPPA ; IN-VITRO ; POLYSACCHARIDES ; PURIFICATION ; EXTRACTION ; DISEASE ; ROOTS |
| 资助项目 | Key Program of National Natural Science Foundation of China[82130111] ; Qi-Huang Chief Scientist Project of National Administration of Traditional Chinese Medicine (2020) ; National Natural Science Foundation of China[82104385] ; Sanming Project of Medicine in Shenzhen[SZZYSM202106004] ; Key-Area Research and Development Program of Guangdong Province[2020B1111110007] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:001056664400001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307031]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China 2.Guangdong Pharmaceut Univ, Coll Pharm, Guangzhou 510006, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhenwei,Wen, Qiuyi,Pi, Jiaju,et al. An inulin-type fructan isolated from Serratula chinensis alleviated the dextran sulfate sodium-induced colitis in mice through regulation of intestinal barrier and gut microbiota[J]. CARBOHYDRATE POLYMERS,2023,320:12. |
| APA | Li, Zhenwei.,Wen, Qiuyi.,Pi, Jiaju.,Zhang, Daidi.,Nie, Jinchun.,...&Guo, De-an.(2023).An inulin-type fructan isolated from Serratula chinensis alleviated the dextran sulfate sodium-induced colitis in mice through regulation of intestinal barrier and gut microbiota.CARBOHYDRATE POLYMERS,320,12. |
| MLA | Li, Zhenwei,et al."An inulin-type fructan isolated from Serratula chinensis alleviated the dextran sulfate sodium-induced colitis in mice through regulation of intestinal barrier and gut microbiota".CARBOHYDRATE POLYMERS 320(2023):12. |
入库方式： OAI收割
来源：上海药物研究所
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