Identification of Specific Peptides in Digested Gelatins by High Performance Liquid Chromatography/Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhang Gui-Feng; Liu Tao; Wang Qian; Lei Jian-Du; Ma Guang-Hui; Su Zhi-Guo |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 36期号:11页码:1499-1504 |
| 关键词 | Gelatin enzymatic digestion marker peptide high performance liquid chromatography/mass spectrometry peptide identification |
| ISSN号 | 0253-3820 |
| 其他题名 | Chin. J. Anal. Chem. |
| 中文摘要 | Sequence analysis of bovine and porcine collagen type I indicates that some partial sequences are specific for bovine or porcine collagen type I, which can be used as marker peptides for gelatin differentiation. Bovine and porcine gelatins were digested with trypsin. The digest mixtures were analyzed with high performance liquid chromatography/mass spectrometry ( HPLC/MS ). Marker peptides were identified by the method of peptide sequence tag. The results indicated that the bovine and porcine. gelatins could be differentiated according to the sequences of marker peptides. Further analysis indicated that some prolines in the marker peptides were hydroxylated, which should be taken into account to increase the confidence in peptide identification. The relative abundance of marker peptides in gelatin of different molecular weight range was also investigated. The results indicate that identification of the marker peptides in the digested gelatins by HPLC/MS is an alternative method for differentiation of bovine and porcine gelatins. |
| 英文摘要 | Sequence analysis of bovine and porcine collagen type I indicates that some partial sequences are specific for bovine or porcine collagen type I, which can be used as marker peptides for gelatin differentiation. Bovine and porcine gelatins were digested with trypsin. The digest mixtures were analyzed with high performance liquid chromatography/mass spectrometry ( HPLC/MS ). Marker peptides were identified by the method of peptide sequence tag. The results indicated that the bovine and porcine. gelatins could be differentiated according to the sequences of marker peptides. Further analysis indicated that some prolines in the marker peptides were hydroxylated, which should be taken into account to increase the confidence in peptide identification. The relative abundance of marker peptides in gelatin of different molecular weight range was also investigated. The results indicate that identification of the marker peptides in the digested gelatins by HPLC/MS is an alternative method for differentiation of bovine and porcine gelatins. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PROLINE HYDROXYLATION ; COLLAGEN ; BOVINE ; DIFFERENTIATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000261465900009 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2907]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Gui-Feng,Liu Tao,Wang Qian,et al. Identification of Specific Peptides in Digested Gelatins by High Performance Liquid Chromatography/Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(11):1499-1504. |
| APA | Zhang Gui-Feng,Liu Tao,Wang Qian,Lei Jian-Du,Ma Guang-Hui,&Su Zhi-Guo.(2008).Identification of Specific Peptides in Digested Gelatins by High Performance Liquid Chromatography/Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(11),1499-1504. |
| MLA | Zhang Gui-Feng,et al."Identification of Specific Peptides in Digested Gelatins by High Performance Liquid Chromatography/Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.11(2008):1499-1504. |
入库方式： OAI收割
来源：过程工程研究所
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