Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)
文献类型：期刊论文
| 作者 | Li, Yanan4 ; Chen, Zongfu4; Zhang, Peng3; Gao, Feng4; Wang, Junfeng2; Lin, Li4; Zhang, Haibin1
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| 刊名 | ANTIOXIDANTS
 |
| 出版日期 | 2023-06-01 |
| 卷号 | 12期号:6页码:15 |
| 关键词 | Holothuroidea deep sea enzyme expression purification |
| DOI | 10.3390/antiox12061227 |
| 通讯作者 | Zhang, Haibin(hzhang@idsse.ac.cn) |
| 英文摘要 | At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 & DEG;C, and it maintains high activity in the range of 0-60 degrees C. It also has high thermal stability when incubated at 37 & DEG;C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products. |
| WOS关键词 | MANGANESE-SUPEROXIDE ; OXIDATIVE STRESS ; REACTIVE OXYGEN ; CLONING ; EXPRESSION ; CDNA |
| 资助项目 | National Natural Science Foundation of China[42006115] ; Guangdong Province Basic and Applied Basic Research Fund Project[2020A1515110826] ; Major Scientific and Technological Projects of the Hainan Province[ZDKJ2021036] ; Guangdong Key Laboratory of Marine Materia Medica[LMM2021-5] ; Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[FEEL-2021-10] ; Key Projects of Guangdong Province College Student Innovation and Entrepreneurship Training Program[S202211347043] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:001016834800001 |
| 资助机构 | National Natural Science Foundation of China ; Guangdong Province Basic and Applied Basic Research Fund Project ; Major Scientific and Technological Projects of the Hainan Province ; Guangdong Key Laboratory of Marine Materia Medica ; Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment ; Key Projects of Guangdong Province College Student Innovation and Entrepreneurship Training Program |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/10380]  |
| 专题 | 深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组 |
| 通讯作者 | Zhang, Haibin |
| 作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China 3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China 4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yanan,Chen, Zongfu,Zhang, Peng,et al. Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)[J]. ANTIOXIDANTS,2023,12(6):15. |
| APA | Li, Yanan.,Chen, Zongfu.,Zhang, Peng.,Gao, Feng.,Wang, Junfeng.,...&Zhang, Haibin.(2023).Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus).ANTIOXIDANTS,12(6),15. |
| MLA | Li, Yanan,et al."Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)".ANTIOXIDANTS 12.6(2023):15. |
入库方式： OAI收割
来源：深海科学与工程研究所
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