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A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians

文献类型：期刊论文

作者Pu, Yujin2,3; Liu, Jun3; Zhang, Nannan1; Liu, Shanshan1; Zhang, Haibin3
刊名MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY
出版日期2023-08-03
页码14
ISSN号1023-6244
关键词DNA extraction deep-sea holothurians high-molecular-weight DNA CTAB >
DOI10.1080/10236244.2023.2241977
通讯作者Zhang, Haibin(hzhang@idsse.ac.cn)
英文摘要With the features as global distribution and all depths dwelling, holothurians function an ideal model for the study of deep-sea adaptation. Acquisition of high-molecular-weight DNA is the basic requirement for molecular biology analyses. However, extraction of high-molecular-weight DNA from deep-sea holothurians is difficult by degradation and contamination. Here, we presented a modified CTAB (lysis buffer with cetyl trimethyl ammonium bromide) method and compared its extraction efficiency for deep-sea holothurians with other five common methods, i.e. the Tiangen Biotech kit, Qiagen kit, Omega Bio-tek kit, Sangon Biotech kit, and SDS (lysis buffer with sodium dodecyl sulfate). Based on the results of yield, integrity, and purity, we found that the modified CTAB method is more suitable to extract high-quality DNA for deep-sea holothurians, with a large amount of yield, minimized degradation, and higher purity. This study provides a high-quality DNA of deep-sea holothurians for genome sequencing and further studies.
WOS关键词ECHINODERMATA HOLOTHUROIDEA ; ELASIPODIDA PSYCHROPOTIDAE ; SPECIES ECHINODERMATA ; MITOCHONDRIAL-DNA ; EXTRACTION ; TISSUES ; STORAGE ; GENUS ; RNA ; DEGRADATION
WOS研究方向Marine & Freshwater Biology
语种英语
出版者TAYLOR & FRANCIS LTD
WOS记录号WOS:001038633900001
源URL[http://ir.idsse.ac.cn/handle/183446/10420]  
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
研究生部
通讯作者Zhang, Haibin
作者单位1.BGI, BGI Qingdao, BGI Shenzhen, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China
推荐引用方式
GB/T 7714		 Pu, Yujin,Liu, Jun,Zhang, Nannan,et al. A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians[J]. MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY,2023:14.
APA Pu, Yujin,Liu, Jun,Zhang, Nannan,Liu, Shanshan,&Zhang, Haibin.(2023).A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians.MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY,14.
MLA Pu, Yujin,et al."A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians".MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY (2023):14.

入库方式： OAI收割

来源：深海科学与工程研究所

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