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10 & mu;m thick ultrathin glass sheet to realize a highly sensitive cantilever for precise cell stiffness measurement

文献类型：期刊论文

作者Yuan, Yapeng4,5; Ma, Doudou4,5; Liu, Xun3; Tang, Tao3; Li, Ming2; Yang, Yang1; Yalikun, Yaxiaer3,4; Tanaka, Yo4,5
刊名LAB ON A CHIP
出版日期2023-07-05
页码11
ISSN号1473-0197
DOI10.1039/d3lc00113j
通讯作者Yalikun, Yaxiaer(yaxiaer@ms.naist.jp) ; Tanaka, Yo(yo.tanaka@a.riken.jp)
英文摘要The micro-cantilever-based sensor platform has become a promising technique in the sensing area for physical, chemical and biological detection due to its portability, small size, label-free characteristics and good compatibility with "lab-on-a-chip" devices. However, traditional micro-cantilever methods are limited by their complicated fabrication, manipulation and detection, and low sensitivity. In this research, we proposed a 10 & mu;m thick ultrathin, highly sensitive, and flexible glass cantilever integrated with a strain gauge sensor and presented its application for the measurement of single-cell mechanical properties. Compared to conventional methods, the proposed ultrathin glass sheet (UTGS)-based cantilever is easier to fabricate, has better physical and chemical properties, and shows a high linear relationship between resistance change and applied small force or displacement. The sensitivity of the cantilever is 15 & mu;N & mu;m(-1) and the minimum detectable displacement at the current development stage is 500 nm, which is sufficient for cell stiffness measurement. The cantilever also possesses excellent optical transparency that supports real-time observation during measurement. We first calibrated the cantilever by measuring the Young's modulus of PDMS with known specific stiffness, and then we demonstrated the measurement of Xenopus oocytes and fertilized eggs in different statuses. By further optimizing the UTGS-based cantilever, we can extend its applicability to various measurements of different cells.
WOS关键词DEFORMABILITY ; DEVICE ; SENSOR
资助项目JSPS[19H05338] ; TEPCO Memorial Foundation ; Amada Foundation ; Japanese Ministry of Education, Culture, Sports, Science, and Technology ; NSG Foundation, Japan ; Tateisi Science and Technology Foundation, Japan ; Iketani Science and Technology Foundation, Japan ; Australian research council (ARC) ; National Health and Medical Research Council Emerging Leadership Fellowship[GNT2017679]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
语种英语
出版者ROYAL SOC CHEMISTRY
WOS记录号WOS:001027771200001
资助机构JSPS ; TEPCO Memorial Foundation ; Amada Foundation ; Japanese Ministry of Education, Culture, Sports, Science, and Technology ; NSG Foundation, Japan ; Tateisi Science and Technology Foundation, Japan ; Iketani Science and Technology Foundation, Japan ; Australian research council (ARC) ; National Health and Medical Research Council Emerging Leadership Fellowship
源URL[http://ir.idsse.ac.cn/handle/183446/10389]  
专题深海工程技术部_深海信息技术研究室
通讯作者Yalikun, Yaxiaer; Tanaka, Yo
作者单位1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China
2.Macquarie Univ, Sch Engn, Sydney 2109, Australia
3.Nara Inst Sci & Technol, Grad Sch, Nara 6300192, Japan
4.RIKEN, Ctr Biosyst Dynam Res BDR, Suita, Osaka 5650871, Japan
5.Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan
推荐引用方式
GB/T 7714		 Yuan, Yapeng,Ma, Doudou,Liu, Xun,et al. 10 & mu;m thick ultrathin glass sheet to realize a highly sensitive cantilever for precise cell stiffness measurement[J]. LAB ON A CHIP,2023:11.
APA Yuan, Yapeng.,Ma, Doudou.,Liu, Xun.,Tang, Tao.,Li, Ming.,...&Tanaka, Yo.(2023).10 & mu;m thick ultrathin glass sheet to realize a highly sensitive cantilever for precise cell stiffness measurement.LAB ON A CHIP,11.
MLA Yuan, Yapeng,et al."10 & mu;m thick ultrathin glass sheet to realize a highly sensitive cantilever for precise cell stiffness measurement".LAB ON A CHIP (2023):11.

入库方式： OAI收割

来源：深海科学与工程研究所

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