Separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo W. T. Wang by HSCCC with a new solvent system screening method
文献类型：期刊论文
| 作者 | Zhang, Shaojun2; Wang, Xiaolei1; Ouyang, Fan1; Su, Zhiguo1; Wang, Changhai2; Gu, Ming1 |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2008 |
| 卷号 | 31期号:17页码:2632-2642 |
| 关键词 | average polarity Corydalis yanhusuo countercurrent chromatography dl-tetrahydropalmatine |
| ISSN号 | 1082-6076 |
| 其他题名 | J. Liq. Chromatogr. Relat. Technol. |
| 中文摘要 | A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high speed countercurrent chromatography separation. Dl-tetrahydropalmatine was separated and purified in one step with n-hexane-ethyl acetate-methanol-water (4-6-5-5) on high speed countercurrent chromatography. Acetic acid was successfully used to adjust the pH value of the sample solution to improve recovery of the separation. As a result, 4.37mg dl-tetrahydropalmatine was separated from 25 mg crude extract at the purity of 92.7% with the recovery of 95.1%. |
| 英文摘要 | A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high speed countercurrent chromatography separation. Dl-tetrahydropalmatine was separated and purified in one step with n-hexane-ethyl acetate-methanol-water (4-6-5-5) on high speed countercurrent chromatography. Acetic acid was successfully used to adjust the pH value of the sample solution to improve recovery of the separation. As a result, 4.37mg dl-tetrahydropalmatine was separated from 25 mg crude extract at the purity of 92.7% with the recovery of 95.1%. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; ALKALOIDS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000258898600009 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2917]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shaojun,Wang, Xiaolei,Ouyang, Fan,et al. Separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo W. T. Wang by HSCCC with a new solvent system screening method[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2008,31(17):2632-2642. |
| APA | Zhang, Shaojun,Wang, Xiaolei,Ouyang, Fan,Su, Zhiguo,Wang, Changhai,&Gu, Ming.(2008).Separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo W. T. Wang by HSCCC with a new solvent system screening method.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,31(17),2632-2642. |
| MLA | Zhang, Shaojun,et al."Separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo W. T. Wang by HSCCC with a new solvent system screening method".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 31.17(2008):2632-2642. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。