Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size
文献类型：期刊论文
| 作者 | Zhou, Qing-Zhu; Wang, Lian-Yan; Ma, Guang-Hui; Su, Zhi-Guo |
| 刊名 | JOURNAL OF MEMBRANE SCIENCE
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 322期号:1页码:98-104 |
| 关键词 | agarose beads uniform-sized premix membrane emulsification chromatography |
| ISSN号 | 0376-7388 |
| 其他题名 | J. Membr. Sci. |
| 中文摘要 | Uniform-sized agarose beads with diameters less than 10 mu m and agarose content as high as 14wt% were prepared by premix membrane emulsification. Agarose aqueous solution was used as the water phase. A mixture of liquid paraffin and petroleum ether containing hexaglycerin penta ester (PO-500) was used as the oil phase. The water phase was mixed with the oil phase at 60 degrees C and a coarse W/O emulsion was produced in a homogenizer. Then, the coarse emulsion was extruded through a hydrophobic membrane under high pressure to form an emulsion, which was slowly cooled under gentle agitation to form gel beads. The effects of preparation conditions on emulsification results were investigated and it showed that the pressure, number of passes, petroleum ether/liquid paraffin (v/v) in the oil phase, the concentration of PO-500 and concentration of agarose in the water phase, all affected the size and uniformity; coarse emulsion did not affect the emulsification results. The coefficient variation (C.V.) of agarose beads under optimal preparation conditions was 9.8%. This method realized microbeads with both uniform sizes and high agarose contents that are difficult to be prepared by conventional emulsion methods. (c) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | Uniform-sized agarose beads with diameters less than 10 mu m and agarose content as high as 14wt% were prepared by premix membrane emulsification. Agarose aqueous solution was used as the water phase. A mixture of liquid paraffin and petroleum ether containing hexaglycerin penta ester (PO-500) was used as the oil phase. The water phase was mixed with the oil phase at 60 degrees C and a coarse W/O emulsion was produced in a homogenizer. Then, the coarse emulsion was extruded through a hydrophobic membrane under high pressure to form an emulsion, which was slowly cooled under gentle agitation to form gel beads. The effects of preparation conditions on emulsification results were investigated and it showed that the pressure, number of passes, petroleum ether/liquid paraffin (v/v) in the oil phase, the concentration of PO-500 and concentration of agarose in the water phase, all affected the size and uniformity; coarse emulsion did not affect the emulsification results. The coefficient variation (C.V.) of agarose beads under optimal preparation conditions was 9.8%. This method realized microbeads with both uniform sizes and high agarose contents that are difficult to be prepared by conventional emulsion methods. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
| 类目[WOS] | Engineering, Chemical ; Polymer Science |
| 研究领域[WOS] | Engineering ; Polymer Science |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; BEADS ; EMULSIONS ; PROTEINS ; GEL |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000259014400013 |
| 公开日期 | 2013-10-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2930]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qing-Zhu,Wang, Lian-Yan,Ma, Guang-Hui,et al. Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size[J]. JOURNAL OF MEMBRANE SCIENCE,2008,322(1):98-104. |
| APA | Zhou, Qing-Zhu,Wang, Lian-Yan,Ma, Guang-Hui,&Su, Zhi-Guo.(2008).Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size.JOURNAL OF MEMBRANE SCIENCE,322(1),98-104. |
| MLA | Zhou, Qing-Zhu,et al."Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size".JOURNAL OF MEMBRANE SCIENCE 322.1(2008):98-104. |
入库方式： OAI收割
来源：过程工程研究所
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