Isolation of lactoferrin from acid whey by magnetic affinity separation
文献类型：期刊论文
| 作者 | Chen, Lin; Guo, Chen; Guan, Yueping; Liu, Huizhou |
| 刊名 | SEPARATION AND PURIFICATION TECHNOLOGY
 |
| 出版日期 | 2007-08-15 |
| 卷号 | 56期号:2页码:168-174 |
| 关键词 | lactoferrin whey magnetic affinity absorbents PGMA |
| ISSN号 | 1383-5866 |
| 其他题名 | Sep. Purif. Technol. |
| 中文摘要 | A micron-sized monodisperse superparamagnetic polyglycidyl methacrylate (PGMA) particles coupled with heparin (PGMA-heparin) was synthesized by the polymerization, and then was evaluated as a method for purifying lactoferrin (LF) from bovine whey. The content of heparin groups at the surface of PGMA particles was 0.92 mg/g. Magnetic PGMA-heparin particles were highly effective in recovering LF directly from acid whey by a single step, whose purity was higher than that of commercial standard proteins, using NaCl solutions as eluent. The maximum LF binding capacity was 164 mg/g. The competitive adsorption tests verified that the magnetic approach offered a fast process and potential at a large scale. The practical use of magnetic PGMA-heparin particles for production of high purified LF from whey is a realistic prospect. (c) 2007 Elsevier B.V. All rights reserved. |
| 英文摘要 | A micron-sized monodisperse superparamagnetic polyglycidyl methacrylate (PGMA) particles coupled with heparin (PGMA-heparin) was synthesized by the polymerization, and then was evaluated as a method for purifying lactoferrin (LF) from bovine whey. The content of heparin groups at the surface of PGMA particles was 0.92 mg/g. Magnetic PGMA-heparin particles were highly effective in recovering LF directly from acid whey by a single step, whose purity was higher than that of commercial standard proteins, using NaCl solutions as eluent. The maximum LF binding capacity was 164 mg/g. The competitive adsorption tests verified that the magnetic approach offered a fast process and potential at a large scale. The practical use of magnetic PGMA-heparin particles for production of high purified LF from whey is a realistic prospect. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | CATION-EXCHANGE CHROMATOGRAPHY ; CHELATOR SUPPORTS ; BINDING ; MILK ; PURIFICATION ; ADSORPTION ; LYSOZYME ; INVIVO |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000248029100006 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3293]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Lin,Guo, Chen,Guan, Yueping,et al. Isolation of lactoferrin from acid whey by magnetic affinity separation[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2007,56(2):168-174. |
| APA | Chen, Lin,Guo, Chen,Guan, Yueping,&Liu, Huizhou.(2007).Isolation of lactoferrin from acid whey by magnetic affinity separation.SEPARATION AND PURIFICATION TECHNOLOGY,56(2),168-174. |
| MLA | Chen, Lin,et al."Isolation of lactoferrin from acid whey by magnetic affinity separation".SEPARATION AND PURIFICATION TECHNOLOGY 56.2(2007):168-174. |
入库方式： OAI收割
来源：过程工程研究所
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