Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption
文献类型：期刊论文
| 作者 | Chen, Wei-Dong; Wang, Yan-Dong; Hu, Han-Hua; Su, Zhi-Guo |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 82期号:2页码:135-142 |
| 关键词 | cation resin hydrodynamics dynamic binding capacity expanded bed adsorption natural product separations |
| ISSN号 | 0268-2575 |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations. |
| 英文摘要 | Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | SUPERCRITICAL-FLUID EXTRACTION ; MONOCLONAL-ANTIBODIES ; PROTEIN ADSORPTION ; PURIFICATION ; CHROMATOGRAPHY ; PERFORMANCE ; EXPANSION ; RECOVERY ; CULTURES ; COLUMN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000244615300003 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3297]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, Duarte, CA 91010 USA 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Wei-Dong,Wang, Yan-Dong,Hu, Han-Hua,et al. Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2007,82(2):135-142. |
| APA | Chen, Wei-Dong,Wang, Yan-Dong,Hu, Han-Hua,&Su, Zhi-Guo.(2007).Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,82(2),135-142. |
| MLA | Chen, Wei-Dong,et al."Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 82.2(2007):135-142. |
入库方式： OAI收割
来源：过程工程研究所
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