Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola
文献类型:期刊论文
作者 | Chen, Wen-Hao; Xu, Chun-Ming; Zeng, Jian-Li; Zhao, Bing; Wang, Xiao-Dong; Wang, Yu-Chun |
刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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出版日期 | 2007-10-01 |
卷号 | 23期号:10页码:1451-1458 |
关键词 | acteoside Cistanche deserticola echinacoside elicitor suspension culture |
ISSN号 | 0959-3993 |
其他题名 | World J. Microbiol. Biotechnol. |
中文摘要 | At appropriate concentrations, polyamines promoted the callus growth and echinacoside content of Cistanche deserticola while Ag+ increased the content of echinacoside and acteoside. In a 20-day culture period, when putrescine (25 mu M) and Ag+ (10 mu M) were added on day 8 and day 16, respectively, the echinacoside production (1.7 g l(-1)) and acteoside production (0.4 g l(-1)) reached the maximum, which were 1.4-fold and 1.5-fold of those in single putrescine treatment, 1.6-fold and 1.4-fold of those in single Ag+ treatment, respectively. Exogenous putrescine enhanced cell viability and antioxidant enzyme activity markedly, so increased the final biomass. Ag+ addition increased H2O2 content and phenylalanine ammonia-lyase activity significantly which led to higher echinacoside and acteoside contents. |
英文摘要 | At appropriate concentrations, polyamines promoted the callus growth and echinacoside content of Cistanche deserticola while Ag+ increased the content of echinacoside and acteoside. In a 20-day culture period, when putrescine (25 mu M) and Ag+ (10 mu M) were added on day 8 and day 16, respectively, the echinacoside production (1.7 g l(-1)) and acteoside production (0.4 g l(-1)) reached the maximum, which were 1.4-fold and 1.5-fold of those in single putrescine treatment, 1.6-fold and 1.4-fold of those in single Ag+ treatment, respectively. Exogenous putrescine enhanced cell viability and antioxidant enzyme activity markedly, so increased the final biomass. Ag+ addition increased H2O2 content and phenylalanine ammonia-lyase activity significantly which led to higher echinacoside and acteoside contents. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | PHENYLETHANOID GLYCOSIDES ; HYDROGEN-PEROXIDE ; TAXOL PRODUCTION ; OXIDATIVE BURST ; POLYAMINES ; ELICITOR ; STRESS ; PLANTS ; BIOSYNTHESIS ; COMBINATION |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000249461900015 |
公开日期 | 2013-10-15 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/3298] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Wen-Hao,Xu, Chun-Ming,Zeng, Jian-Li,et al. Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2007,23(10):1451-1458. |
APA | Chen, Wen-Hao,Xu, Chun-Ming,Zeng, Jian-Li,Zhao, Bing,Wang, Xiao-Dong,&Wang, Yu-Chun.(2007).Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,23(10),1451-1458. |
MLA | Chen, Wen-Hao,et al."Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 23.10(2007):1451-1458. |
入库方式: OAI收割
来源:过程工程研究所
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