Microwave-assisted extraction of scutellarin from Erigeron breviscapus Hand-Mazz and its determination by high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Gao, Min; Huang, Wei; RoyChowdhury, Moytri; Liu, Chunzhao |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2007-05-22 |
| 卷号 | 591期号:2页码:161-166 |
| 关键词 | Erigeron breviscapus heat-reflux extraction high-performance liquid chromatography microwave-assisted extraction scutellarin |
| ISSN号 | 0003-2670 |
| 其他题名 | Anal. Chim. Acta |
| 中文摘要 | An efficient microwave-assisted extraction (MAE) technique has been developed to extract scutellarin from Erigeron breviscapus for rapid determination by high-performance liquid chromatography (HPLC). The maximum yield of scutellarin reached 1.02% in 40 min under the optimal MAE conditions with 80 degrees C of extraction temperature and 1: 10 (w/v) of the solid/liquid ratio. The MAE showed obvious advantages in terms of short duration and high efficiency to extract scutellarin in comparison with heat-flux extraction. The mechanism of the enhanced extraction by microwave assistance was discussed by detecting particle size and specific surface area of plant materials and observing cell destruction of plant material by light microscopy and scanning electron microscopy. The results showed that the plant materials were significantly destroyed due to the cell rupture after MAE treatment. Afterward, the method validation for HPLC-UV analysis was developed. Calibration range was 0.1-100 mu g mL(-1) for scutellarin, and correlation coefficient R was 0.9993. Limit of detection was less than 0.0 1 mu g mL(-1). The intra- and inter-day relative standard deviation (R.S.D.) of scutellarin detection ranged from 1.58% to 2.96% and from 3.32% to 4.19%, respectively. The recovery of the method for scutellarin ranged from 96.7% to 101.9%. (C) 2007 Published by Elsevier B.V. |
| 英文摘要 | An efficient microwave-assisted extraction (MAE) technique has been developed to extract scutellarin from Erigeron breviscapus for rapid determination by high-performance liquid chromatography (HPLC). The maximum yield of scutellarin reached 1.02% in 40 min under the optimal MAE conditions with 80 degrees C of extraction temperature and 1: 10 (w/v) of the solid/liquid ratio. The MAE showed obvious advantages in terms of short duration and high efficiency to extract scutellarin in comparison with heat-flux extraction. The mechanism of the enhanced extraction by microwave assistance was discussed by detecting particle size and specific surface area of plant materials and observing cell destruction of plant material by light microscopy and scanning electron microscopy. The results showed that the plant materials were significantly destroyed due to the cell rupture after MAE treatment. Afterward, the method validation for HPLC-UV analysis was developed. Calibration range was 0.1-100 mu g mL(-1) for scutellarin, and correlation coefficient R was 0.9993. Limit of detection was less than 0.0 1 mu g mL(-1). The intra- and inter-day relative standard deviation (R.S.D.) of scutellarin detection ranged from 1.58% to 2.96% and from 3.32% to 4.19%, respectively. The recovery of the method for scutellarin ranged from 96.7% to 101.9%. (C) 2007 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | LIGHT-SCATTERING DETECTION ; SAUSSUREA-MEDUSA MAXIM ; HYDROGEN-PEROXIDE ; CULTURED-CELLS ; OPTIMIZATION ; FLAVONOIDS ; SOLANESOL ; TOBACCO |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000246730500004 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3313]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Phytochem Engn Grp, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Beijing Res Inst Nutr Resources, Beijing 100069, Peoples R China 3.Arkansas State Univ, Arkansas Biosci Inst, Jonesboro, AR 72401 USA 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Min,Huang, Wei,RoyChowdhury, Moytri,et al. Microwave-assisted extraction of scutellarin from Erigeron breviscapus Hand-Mazz and its determination by high-performance liquid chromatography[J]. ANALYTICA CHIMICA ACTA,2007,591(2):161-166. |
| APA | Gao, Min,Huang, Wei,RoyChowdhury, Moytri,&Liu, Chunzhao.(2007).Microwave-assisted extraction of scutellarin from Erigeron breviscapus Hand-Mazz and its determination by high-performance liquid chromatography.ANALYTICA CHIMICA ACTA,591(2),161-166. |
| MLA | Gao, Min,et al."Microwave-assisted extraction of scutellarin from Erigeron breviscapus Hand-Mazz and its determination by high-performance liquid chromatography".ANALYTICA CHIMICA ACTA 591.2(2007):161-166. |
入库方式： OAI收割
来源：过程工程研究所
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