One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Gu, Ming; Wang, Xiaolei; Su, Zhiguo; Fan Ouyang |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2007-01-26 |
| 卷号 | 1140期号:1-2页码:107-111 |
| 关键词 | high-speed counter-current chromatography polyphenol 3 salvianolic acid B protocatechualdehyde 4-dihydroxyphenyllactic acid |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Three kinds of polyphenols of Salvia miltiorrhiza Bunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde, were separated and purified in one step with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1.5:8) by high-speed countercurrent chromatography. Acetic acid was successfully used to increase the partition of high polar target compounds in organic phase to modify partition coefficient value. 3,4-Dihydroxyphenyl lactic acid, salvianolic acid B and protocatechualdehyde were purified from 100 mg water extracted crude sample of Salvia miltiorrhiza Bunge at purity of 97.6%, 94.2% and 98.2% and at yield of 98.6%, 73.6% and 90.2%. High-speed countercurrent chromatography together with organic/aqueous solvent system supplied an efficient method to purify water-soluble compounds directly from crude samples of traditional Chinese medicines. (c) 2006 Elsevier B.V All rights reserved. |
| 英文摘要 | Three kinds of polyphenols of Salvia miltiorrhiza Bunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde, were separated and purified in one step with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1.5:8) by high-speed countercurrent chromatography. Acetic acid was successfully used to increase the partition of high polar target compounds in organic phase to modify partition coefficient value. 3,4-Dihydroxyphenyl lactic acid, salvianolic acid B and protocatechualdehyde were purified from 100 mg water extracted crude sample of Salvia miltiorrhiza Bunge at purity of 97.6%, 94.2% and 98.2% and at yield of 98.6%, 73.6% and 90.2%. High-speed countercurrent chromatography together with organic/aqueous solvent system supplied an efficient method to purify water-soluble compounds directly from crude samples of traditional Chinese medicines. (c) 2006 Elsevier B.V All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PREPARATIVE ISOLATION ; ROSMARINIC ACID ; DITERPENOIDS ; TANSHINONES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000243953300014 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3318]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Ming,Wang, Xiaolei,Su, Zhiguo,et al. One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1140(1-2):107-111. |
| APA | Gu, Ming,Wang, Xiaolei,Su, Zhiguo,&Fan Ouyang.(2007).One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1140(1-2),107-111. |
| MLA | Gu, Ming,et al."One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1140.1-2(2007):107-111. |
入库方式： OAI收割
来源：过程工程研究所
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