Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9
文献类型：期刊论文
| 作者 | Li AQ(李安琦) ; Wei-Jia Zhang; Xue-Gong Li; Xu-Chong Bao; Xiao-Qing Qi; Long-Fei Wu; Douglas H. Bartlett
|
| 刊名 | Microorganisms
 |
| 出版日期 | 2023-03-02 |
| 卷号 | 11页码:637 |
| 关键词 | deep-sea bacterium high hydrostatic pressure piezophilic ATPase energy metabolism |
| 通讯作者 | Wei-Jia Zhang |
| 英文摘要 | Abstract: Alteration of respiratory components as a function of pressure is a common strategy developed in deep-sea microorganisms, presumably to adapt to high hydrostatic pressure (HHP). While the electron transport chain and terminal reductases have been extensively studied in deep-sea bacteria, little is known about their adaptations for ATP generation. In this study, we showed
that the deep-sea bacterium Photobacterium profundum SS9 exhibits a more pronounced piezophilic phenotype when grown in minimal medium supplemented with glucose (MG) than in the routinely used MB2216 complex medium. The intracellular ATP level varied with pressure, but with opposite
trends in the two culture media. Between the two ATPase systems encoded in SS9, ATPase-I played a dominant role when cultivated in MB2216, whereas ATPase-II was more abundant in the MG medium, especially at elevated pressure when cells had the lowest ATP level among all conditions tested.
Further analyses of the ∆atpI, ∆atpE1 and ∆atpE2 mutants showed that disrupting ATPase-I induced expression of ATPase-II and that the two systems are functionally redundant in MB2216. Collectively, we provide the first examination of the differences and relationships between two ATPase systems in
a piezophilic bacterium, and expanded our understanding of the involvement of energy metabolism in pressure adaptation. |
| 语种 | 英语 |
| 版本 | 出版稿 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/10516]  |
| 专题 | 深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组 |
| 通讯作者 | Wei-Jia Zhang |
| 作者单位 | 1.中国科学院深海科学与工程研究所 2.中国科学院海洋研究所 3.中国科学院大学 |
| 推荐引用方式 GB/T 7714 | Li AQ,Wei-Jia Zhang,Xue-Gong Li,et al. Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9[J]. Microorganisms,2023,11:637. |
| APA | Li AQ.,Wei-Jia Zhang.,Xue-Gong Li.,Xu-Chong Bao.,Xiao-Qing Qi.,...&Douglas H. Bartlett.(2023).Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9.Microorganisms,11,637. |
| MLA | Li AQ,et al."Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9".Microorganisms 11(2023):637. |
入库方式： OAI收割
来源：深海科学与工程研究所
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