Enzymatic hydrolysis of penicillin in mixed ionic Liquids/Water two-phase system
文献类型：期刊论文
| 作者 | Jiang, Y. Y. ; Xia, H. S. ; Guo, C. ; Mahmood, I. ; Liu, H. Z. |
| 刊名 | Biotechnology Progress
 |
| 出版日期 | 2007 |
| 期号 | 4页码:829-835 |
| 关键词 | potassium phosphate solutions acylase-catalyzed synthesis beta-lactam antibiotics 6-aminopenicillanic acid poly(ethylene glycol) organic cosolvents water activity salt-solutions low ph extraction |
| 其他题名 | Biotechnol. Prog. |
| 中文摘要 | In this paper, an integrated process involving the mixed ionic liquids/water two-phase system (MILWS) is proposed to improve the efficiency for enzymatic hydrolysis of penicillin G. First, hydrophilic [C(4)mim]BF4 (1-butyl- 3-methylimidazolium tetrafluoraborate) and NaH2PO4 salt form an ionic liquids aqueous two-phase system (ILATPS), which could extract penicillin from its fermentation broth efficiently. Second, a hydrophobic [C(4)mim]PF6 (1-butyl-3-methylimidazolium hexafluoraphosphate) is introduced into the ionic liquids-rich phase of ILATPS containing penicillin and converses it into MILWS. Penicillin is hydrolyzed by penicillin acylase in the water phase of MILWS at pH5. The byproduct phenylacetic acid (PAA) is partitioned into the ionic liquids mixture phase, while the intended product 6-aminopenicillanic acid (6-APA) is precipitated at this pH. In comparison with a similar butyl acetate/water system (BAWS) at pH 4, MILWS exhibits two advantages. (1) The selectivity between PAA and penicillin is greatly optimized at pH 5 by varying the mole ratio Of [C(4)mim]PF6/[C(4)mim]BF4 in MILWS, whereas in BAWS the unalterable nature of the organic solvent restricts the optimized pH for maximum selectivity between PAA and penicillin at pH 4. (2) The pH for 6-APA precipitation in BAWS is 4, whereas it shifts to pH 5 in MILWS due to the complexation between negatively charged 6-APA and the cationic surface of the ionic liquids micelle. As a result, the removal of the two products from the enzyme sphere at relatively high pH is permitted in MILWS, which is beneficial for enzymatic activity and stability in comparison with the acidic pH 4 environment in BAWS. |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3352]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Jiang, Y. Y.,Xia, H. S.,Guo, C.,et al. Enzymatic hydrolysis of penicillin in mixed ionic Liquids/Water two-phase system[J]. Biotechnology Progress,2007(4):829-835. |
| APA | Jiang, Y. Y.,Xia, H. S.,Guo, C.,Mahmood, I.,&Liu, H. Z..(2007).Enzymatic hydrolysis of penicillin in mixed ionic Liquids/Water two-phase system.Biotechnology Progress(4),829-835. |
| MLA | Jiang, Y. Y.,et al."Enzymatic hydrolysis of penicillin in mixed ionic Liquids/Water two-phase system".Biotechnology Progress .4(2007):829-835. |
入库方式： OAI收割
来源：过程工程研究所
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