An effective method for fast determination of artemisinin in Artemisia annua L. by high performance liquid chromatography with evaporative light scattering detection
文献类型：期刊论文
| 作者 | Liu, Chun-Zhao; Zhou, Hua-Ying; Zhao, Yan |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2007-01-09 |
| 卷号 | 581期号:2页码:298-302 |
| 关键词 | evaporative light scattering detection high performance liquid chromatography microwave-assisted extraction artemisinin Artemisia annua L |
| ISSN号 | 0003-2670 |
| 其他题名 | Anal. Chim. Acta |
| 中文摘要 | Artemisinin isolated from the aerial parts of Artemisia annua L., is a promising and potent antimalarial drug, which meets the dual challenge posed by drug-resistant parasites and rapid progression of malarial illness. The aim of the current study was to develop a reliable and fast analytical procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. The HPLC conditions were Agilent C18 column using water:acetonitrile (40:60 v/v) mixture as mobile phase at a flow rate of 1 mL min(-1). ELSD conditions were optimized at nebulizer-gas flow rate of 2.0 L min(-1) and drift tube temperature of 70 degrees C under the impactor off-mode, and the gain was set at 2. Afterwards, method validation system for HPLC-ELSD analysis was developed. Calibration range was 0.2-1.0 mg mL(-1) and correlation coefficient r was above 0.9990. Precision experiments showed relative standard deviation (R.S.D.) of retention time was less than 0.5% and R.S.D. of peak area was less than 1.30%. Inter-day and intra-day variabilities showed that R.S.D. was ranged from 1.01% to 4.66%. Limit of detection was less than 40 mu g mL(-1) and limit of quantification was less than 100 jig mL-'. Accuracy validation showed that average recovery was between 98.23% and 104.97%. The developed analytical procedure was successfully applied to determine the contents of artemisinin in the different parts of A. annua plants. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Artemisinin isolated from the aerial parts of Artemisia annua L., is a promising and potent antimalarial drug, which meets the dual challenge posed by drug-resistant parasites and rapid progression of malarial illness. The aim of the current study was to develop a reliable and fast analytical procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. The HPLC conditions were Agilent C18 column using water:acetonitrile (40:60 v/v) mixture as mobile phase at a flow rate of 1 mL min(-1). ELSD conditions were optimized at nebulizer-gas flow rate of 2.0 L min(-1) and drift tube temperature of 70 degrees C under the impactor off-mode, and the gain was set at 2. Afterwards, method validation system for HPLC-ELSD analysis was developed. Calibration range was 0.2-1.0 mg mL(-1) and correlation coefficient r was above 0.9990. Precision experiments showed relative standard deviation (R.S.D.) of retention time was less than 0.5% and R.S.D. of peak area was less than 1.30%. Inter-day and intra-day variabilities showed that R.S.D. was ranged from 1.01% to 4.66%. Limit of detection was less than 40 mu g mL(-1) and limit of quantification was less than 100 jig mL-'. Accuracy validation showed that average recovery was between 98.23% and 104.97%. The developed analytical procedure was successfully applied to determine the contents of artemisinin in the different parts of A. annua plants. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MICROWAVE-ASSISTED EXTRACTION ; GINKGO-BILOBA ; OPTIMIZATION ; VALIDATION ; HPLC ; ELSD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000243665200013 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3387]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chun-Zhao,Zhou, Hua-Ying,Zhao, Yan. An effective method for fast determination of artemisinin in Artemisia annua L. by high performance liquid chromatography with evaporative light scattering detection[J]. ANALYTICA CHIMICA ACTA,2007,581(2):298-302. |
| APA | Liu, Chun-Zhao,Zhou, Hua-Ying,&Zhao, Yan.(2007).An effective method for fast determination of artemisinin in Artemisia annua L. by high performance liquid chromatography with evaporative light scattering detection.ANALYTICA CHIMICA ACTA,581(2),298-302. |
| MLA | Liu, Chun-Zhao,et al."An effective method for fast determination of artemisinin in Artemisia annua L. by high performance liquid chromatography with evaporative light scattering detection".ANALYTICA CHIMICA ACTA 581.2(2007):298-302. |
入库方式： OAI收割
来源：过程工程研究所
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