Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting
文献类型：期刊论文
| 作者 | Tao, Liu; Zhang Gui-Feng; Zhou Wei-Bin; Su Zhi-Guo |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2007 |
| 卷号 | 35期号:1页码:43-48 |
| 关键词 | succinylated gelatin modification degree size exclusion chromatography multi angle laser light scatting trinitrobenzenesulfonic acid |
| ISSN号 | 0253-3820 |
| 其他题名 | Chin. J. Anal. Chem. |
| 中文摘要 | The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin. |
| 英文摘要 | The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | 2,4,6-TRINITROBENZENESULFONIC ACID ; SCATTERING ; AMINES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000246604700008 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3445]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Liu,Zhang Gui-Feng,Zhou Wei-Bin,et al. Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(1):43-48. |
| APA | Tao, Liu,Zhang Gui-Feng,Zhou Wei-Bin,&Su Zhi-Guo.(2007).Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(1),43-48. |
| MLA | Tao, Liu,et al."Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.1(2007):43-48. |
入库方式： OAI收割
来源：过程工程研究所
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