Neuroprotective effects of safflor yellow B on brain ischemic injury
文献类型：期刊论文
| 作者 | Wang, Chaoyun; Zhang, Dalei; Li, Guisheng; Liu, Juntian; Tian, Jingwei; Fu, Fenghua; Liu, Ke |
| 刊名 | EXPERIMENTAL BRAIN RESEARCH
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 177期号:4页码:533-539 |
| 关键词 | safflor yellow B cerebral ischemia neuroprotection free radicals glutamate energy metabolism |
| ISSN号 | 0014-4819 |
| 其他题名 | Exp. Brain Res. |
| 中文摘要 | The present study was conducted to investigate whether safflor yellow B (SYB) had a protective effect on cerebral ischemic injury and to determine the possible mechanisms in vivo and in vitro. In vivo, Male Wistar-Kyoto (WKY) rats were used to make the model of middle cerebral artery occlusion (MCAO). The behavioral test was used to measure neurological deficit scores for evaluation of the ischemic damage of brain. The infarction area of brain was assessed in brain slices stained with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain. Furthermore, the respiratory control ratio (RCR = state 3/state 4) was assessed in the brain mitochondria. In vitro, the effect of SYB was tested in cultured fetal cortical cells exposed to glutamate to identify its neuroprotection against neurons damage. The results in vivo showed that SYB at doses of 3.0 and 6.0 mg kg(-1) markedly decreased the neurological deficit scores and the infarction area in MCAO rats. At the same time, SYB significantly improved mitochondrial energy metabolism, decreased MDA content, and increased SOD and GPx activities in ischemic brain. The results in vitro showed that SYB remarkably inhibited neuron damage induced by glutamate in cultured fetal cortical cells. These suggest that SYB might act as a potential neuroprotective agent against the cerebral ischemia-induced injury in rat brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism. |
| 英文摘要 | The present study was conducted to investigate whether safflor yellow B (SYB) had a protective effect on cerebral ischemic injury and to determine the possible mechanisms in vivo and in vitro. In vivo, Male Wistar-Kyoto (WKY) rats were used to make the model of middle cerebral artery occlusion (MCAO). The behavioral test was used to measure neurological deficit scores for evaluation of the ischemic damage of brain. The infarction area of brain was assessed in brain slices stained with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain. Furthermore, the respiratory control ratio (RCR = state 3/state 4) was assessed in the brain mitochondria. In vitro, the effect of SYB was tested in cultured fetal cortical cells exposed to glutamate to identify its neuroprotection against neurons damage. The results in vivo showed that SYB at doses of 3.0 and 6.0 mg kg(-1) markedly decreased the neurological deficit scores and the infarction area in MCAO rats. At the same time, SYB significantly improved mitochondrial energy metabolism, decreased MDA content, and increased SOD and GPx activities in ischemic brain. The results in vitro showed that SYB remarkably inhibited neuron damage induced by glutamate in cultured fetal cortical cells. These suggest that SYB might act as a potential neuroprotective agent against the cerebral ischemia-induced injury in rat brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Neurosciences |
| 研究领域[WOS] | Neurosciences & Neurology |
| 关键词[WOS] | CEREBRAL-ISCHEMIA ; GLUTAMATE RELEASE ; NEURONAL INJURY ; RAT-BRAIN ; CALCIUM ; DEPRIVATION ; REPERFUSION ; ACCUMULATION ; METABOLISM ; APOPTOSIS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000244483600010 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3449]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Xian Jiaotong Univ, Sch Med, Dept Pharmacol, Xian 710061, Peoples R China 2.Xian Jiaotong Univ, Key Lab Environm & Genes Related Dis, Minist Educ, Xian 710061, Peoples R China 3.Shandong Engn Res Ctr Nat Drug, Shandong 264005, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chaoyun,Zhang, Dalei,Li, Guisheng,et al. Neuroprotective effects of safflor yellow B on brain ischemic injury[J]. EXPERIMENTAL BRAIN RESEARCH,2007,177(4):533-539. |
| APA | Wang, Chaoyun.,Zhang, Dalei.,Li, Guisheng.,Liu, Juntian.,Tian, Jingwei.,...&Liu, Ke.(2007).Neuroprotective effects of safflor yellow B on brain ischemic injury.EXPERIMENTAL BRAIN RESEARCH,177(4),533-539. |
| MLA | Wang, Chaoyun,et al."Neuroprotective effects of safflor yellow B on brain ischemic injury".EXPERIMENTAL BRAIN RESEARCH 177.4(2007):533-539. |
入库方式： OAI收割
来源：过程工程研究所
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