Neuroprotective effects of emodin-8-O-beta-D-glucoside in vivo and in vitro
文献类型：期刊论文
| 作者 | Wang, Chaoyun; Zhang, Dalei; Ma, Hongmei; Liu, Juntian |
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2007-12-22 |
| 卷号 | 577期号:1-3页码:58-63 |
| 关键词 | Polygonum cuspidatum Sieb. et Zucc emodin-8-O-beta-D-glucoside ischemia and reperfusion neuroprotection lipid peroxidation |
| ISSN号 | 0014-2999 |
| 其他题名 | Eur. J. Pharmacol. |
| 中文摘要 | Emodin-8-O-beta-D-glucoside extracted from the traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc is widely used to treat acute hepatitis possibly by antioxidative mechanisms. The present study was designed to investigate whether emodin-8-O-beta-D-glucoside exerted neuroprotective effects on the focal cerebral injury induced by ischemia and reperfusion in vivo and on the neuronal damage induced by glutamate in vitro, and to study the possible mechanisms. Male Wistar rats were used to establish the model of ischemia and reperfusion. The behavioral test was performed and the cerebral infarction area was assessed in the brain slices stained with 2% 2,3,5-triphenyl tetrazolium chloride to evaluate the neuroprotective effects of emodin-8-O-beta-D-glucoside. Superoxide dismutase (SOD) activity, total antioxidative capability and malondialdehyde (MDA) level in the brain tissue were determined with spectrophotometrical methods to probe the primary mechanisms of emodin-8-O-beta-D-glucoside. In vitro, the neuroprotective effects of emodin-8-O-beta-D-glucoside were tested in the cultured cortical cells of fetal rats exposed to glutamate. Emodin-8-O-beta-D-glucoside concentration in plasma and brain tissue was also measured to examine distribution of emodin-8-O- -D-glucoside in the brain. The results showed that the treatment of rats with emodin-8-O-beta-D-glucoside reduced the neurological deficit score and the cerebral infarction area, increased SOD activity and total antioxidative capability, and decreased MDA level in the brain tissue in dose-dependent way. Emodm-8-O-beta-D-glucoside also inhibited the neuronal damage induced by glutamate. Besides, emodin-8-O-beta-D-glucoside was able to penetrate blood-brain barrier and distribute in the brain tissue. These findings demonstrate that emodin-8-O-beta-D-glucoside is able to provide neuroprotection against cerebral ischemia-reperfused injury and glutamate induced neuronal damage through exerting antioxidative effects and inhibiting glutamate neurotoxicity. (c) 2007 Elsevier B.V All rights reserved. |
| 英文摘要 | Emodin-8-O-beta-D-glucoside extracted from the traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc is widely used to treat acute hepatitis possibly by antioxidative mechanisms. The present study was designed to investigate whether emodin-8-O-beta-D-glucoside exerted neuroprotective effects on the focal cerebral injury induced by ischemia and reperfusion in vivo and on the neuronal damage induced by glutamate in vitro, and to study the possible mechanisms. Male Wistar rats were used to establish the model of ischemia and reperfusion. The behavioral test was performed and the cerebral infarction area was assessed in the brain slices stained with 2% 2,3,5-triphenyl tetrazolium chloride to evaluate the neuroprotective effects of emodin-8-O-beta-D-glucoside. Superoxide dismutase (SOD) activity, total antioxidative capability and malondialdehyde (MDA) level in the brain tissue were determined with spectrophotometrical methods to probe the primary mechanisms of emodin-8-O-beta-D-glucoside. In vitro, the neuroprotective effects of emodin-8-O-beta-D-glucoside were tested in the cultured cortical cells of fetal rats exposed to glutamate. Emodin-8-O-beta-D-glucoside concentration in plasma and brain tissue was also measured to examine distribution of emodin-8-O- -D-glucoside in the brain. The results showed that the treatment of rats with emodin-8-O-beta-D-glucoside reduced the neurological deficit score and the cerebral infarction area, increased SOD activity and total antioxidative capability, and decreased MDA level in the brain tissue in dose-dependent way. Emodm-8-O-beta-D-glucoside also inhibited the neuronal damage induced by glutamate. Besides, emodin-8-O-beta-D-glucoside was able to penetrate blood-brain barrier and distribute in the brain tissue. These findings demonstrate that emodin-8-O-beta-D-glucoside is able to provide neuroprotection against cerebral ischemia-reperfused injury and glutamate induced neuronal damage through exerting antioxidative effects and inhibiting glutamate neurotoxicity. (c) 2007 Elsevier B.V All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; ANTIOXIDANT DEFENSE ; OXIDATIVE STRESS ; NEURONAL INJURY ; BRAIN ISCHEMIA ; NITRIC-OXIDE ; MECHANISMS ; REPERFUSION ; RECEPTORS ; OCCLUSION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000251155000008 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3450]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Xian Jiaotong Univ, Sch Med, Xian 710061, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.China Agr Univ, Yantai 264002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chaoyun,Zhang, Dalei,Ma, Hongmei,et al. Neuroprotective effects of emodin-8-O-beta-D-glucoside in vivo and in vitro[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2007,577(1-3):58-63. |
| APA | Wang, Chaoyun,Zhang, Dalei,Ma, Hongmei,&Liu, Juntian.(2007).Neuroprotective effects of emodin-8-O-beta-D-glucoside in vivo and in vitro.EUROPEAN JOURNAL OF PHARMACOLOGY,577(1-3),58-63. |
| MLA | Wang, Chaoyun,et al."Neuroprotective effects of emodin-8-O-beta-D-glucoside in vivo and in vitro".EUROPEAN JOURNAL OF PHARMACOLOGY 577.1-3(2007):58-63. |
入库方式： OAI收割
来源：过程工程研究所
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