Preparation and application of novel microspheres possessing autofluorescent properties
文献类型：期刊论文
| 作者 | Wei, Wei; Wang, Lian-Yan; Yuan, Lan; Wei, Qiang; Yang, Xiao-Da; Su, Zhi-Guo; Ma, Guang-Hui |
| 刊名 | ADVANCED FUNCTIONAL MATERIALS
 |
| 出版日期 | 2007-11-05 |
| 卷号 | 17期号:16页码:3153-3158 |
| 关键词 | fluorescent latex microspheres blood-flow distribution nanoparticles nanospheres |
| ISSN号 | 1616-301X |
| 其他题名 | Adv. Funct. Mater. |
| 中文摘要 | Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The auto-fluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers. |
| 英文摘要 | Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The auto-fluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 研究领域[WOS] | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 关键词[WOS] | FLUORESCENT LATEX MICROSPHERES ; BLOOD-FLOW DISTRIBUTION ; NANOPARTICLES ; NANOSPHERES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000250989600017 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3474]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China 4.Peking Univ, Hlth Sci Ctr, State Key Labs Nat & Biomimet Drugs, Dept Biol Chem, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wei,Wang, Lian-Yan,Yuan, Lan,et al. Preparation and application of novel microspheres possessing autofluorescent properties[J]. ADVANCED FUNCTIONAL MATERIALS,2007,17(16):3153-3158. |
| APA | Wei, Wei.,Wang, Lian-Yan.,Yuan, Lan.,Wei, Qiang.,Yang, Xiao-Da.,...&Ma, Guang-Hui.(2007).Preparation and application of novel microspheres possessing autofluorescent properties.ADVANCED FUNCTIONAL MATERIALS,17(16),3153-3158. |
| MLA | Wei, Wei,et al."Preparation and application of novel microspheres possessing autofluorescent properties".ADVANCED FUNCTIONAL MATERIALS 17.16(2007):3153-3158. |
入库方式： OAI收割
来源：过程工程研究所
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