A Nanopore Sensing Assay Resolves Cascade Reactions in a Multienzyme System
文献类型：期刊论文
| 作者 | Sheng, Yingying,; Zhou, Ke; Liu, Lei ; Wu, Hai-Chen,
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| 刊名 | Angewandte Chemie - International Edition
 |
| 出版日期 | 2022 |
| 卷号 | 61期号:20页码:e202200866 |
| ISSN号 | 14337851 |
| DOI | 10.1002/anie.202200866 |
| 文献子类 | Article |
| 英文摘要 | Enzymatic cascade reactions are widely used to synthesize complex molecules from simple precursors. The major underlying mechanism of cascade reactions is substrate channelling, where intermediates of different enzymatic steps are not in equilibrium with the bulk solution. Here, we report a nanopore sensing assay that allows accurate quantification of all the reaction intermediates and the product of an artificial three-enzyme system. A DNA–peptide complex is used as the initial substrate which undergoes sequential enzymatic cleavages in solution. All the temporal changes of the intermediates and product can be obtained through nanopore translocation recordings. Furthermore, we find that in a confined environment such as liposome, substrate channelling occurs between two sets of the three enzymes. Our results demonstrate a novel and powerful approach to determine and quantify substrate channelling effects, which is potentially useful for designing and evaluating multienzyme systems. © 2022 Wiley-VCH GmbH. |
| 电子版国际标准刊号 | 15213773 |
| 语种 | 英语 |
| WOS记录号 | WOS:000769510000001 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/299166]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_东莞分部 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Sheng, Yingying,,Zhou, Ke,Liu, Lei,et al. A Nanopore Sensing Assay Resolves Cascade Reactions in a Multienzyme System[J]. Angewandte Chemie - International Edition,2022,61(20):e202200866. |
| APA | Sheng, Yingying,,Zhou, Ke,Liu, Lei,&Wu, Hai-Chen,.(2022).A Nanopore Sensing Assay Resolves Cascade Reactions in a Multienzyme System.Angewandte Chemie - International Edition,61(20),e202200866. |
| MLA | Sheng, Yingying,,et al."A Nanopore Sensing Assay Resolves Cascade Reactions in a Multienzyme System".Angewandte Chemie - International Edition 61.20(2022):e202200866. |
入库方式： OAI收割
来源：高能物理研究所
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