Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa
文献类型:期刊论文
作者 | Xu, Chunming; Zhao, Bing; Ou, Yuan; Wang, Xiaodong; Yuan, Xiaofan; Wang, Yuchun |
刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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出版日期 | 2007-07-01 |
卷号 | 23期号:7页码:965-970 |
关键词 | elicitor G6PDH PAL POD Saussurea medusa syringin suspension culture |
ISSN号 | 0959-3993 |
其他题名 | World J. Microbiol. Biotechnol. |
中文摘要 | Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag+) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5% (v/v) yeast extract, 0.2 g l(-1) chitosan and 75 mu M Ag+ at the 15th day of the cell culture. The highest syringin production reached 741.9 mg l(-1), which was 3.6-fold that of the control. The glucose-6-phosphate dehydrogenase (EC 1.1.1.49), phenylalanine ammonia lyase (EC 4.3.1.5) and peroxidase (EC 1.11.1.7) activities increased significantly after elicitor treatment. The maximum enzyme activities were obtained when the treatment time was 6 h. |
英文摘要 | Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag+) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5% (v/v) yeast extract, 0.2 g l(-1) chitosan and 75 mu M Ag+ at the 15th day of the cell culture. The highest syringin production reached 741.9 mg l(-1), which was 3.6-fold that of the control. The glucose-6-phosphate dehydrogenase (EC 1.1.1.49), phenylalanine ammonia lyase (EC 4.3.1.5) and peroxidase (EC 1.11.1.7) activities increased significantly after elicitor treatment. The maximum enzyme activities were obtained when the treatment time was 6 h. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | PENTOSE-PHOSPHATE PATHWAY ; PACLITAXEL PRODUCTION ; TAXUS-CHINENSIS ; ACTIVATION ; JACEOSIDIN ; DEFENSE ; GROWTH ; PLANTS ; CELLS ; ROOT |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000247392800009 |
公开日期 | 2013-10-15 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/3483] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Chunming,Zhao, Bing,Ou, Yuan,et al. Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2007,23(7):965-970. |
APA | Xu, Chunming,Zhao, Bing,Ou, Yuan,Wang, Xiaodong,Yuan, Xiaofan,&Wang, Yuchun.(2007).Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,23(7),965-970. |
MLA | Xu, Chunming,et al."Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 23.7(2007):965-970. |
入库方式: OAI收割
来源:过程工程研究所
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